2 2 No Abstract Editorial 60577.pdf ####

The Role of Biotechnology in Latest Therapeutic Approaches for Diabetes Mellitus 2 2 No Abstract 66 67 Sepideh Hajivalizadeh Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences Iran Shahin Akhondzadeh Artificial pancreasGastrointestinal microbiomeGlucoseInsulinMonoclonal antibodyStem cell transplantationTissue engineering Treatment 60568.pdf Elnashar M, Vaccarezza M, Al-Salami H. Cutting-edge biotechnological advancement in islet delivery using pancreatic and cellular approaches. Future Sci OA 2020 Nov 23;7(3):FSO660.##Reyes-Martínez JE, Ruiz-Pacheco JA, Flores-Valdéz MA, Elsawy MA, Vallejo-Cardona AA, Castillo-Díaz LA. Advanced hydrogels for treatment of diabetes. J Tissue Eng Regen Med 2019 Aug;13(8):1375-93.##Davies M, Dahl D, Heise T, Kiljanski J, Mathieu C. Introduction of biosimilar insulins in Europe. Diabet Med J Br Diabet Assoc 2017 Oct;34(10):1340-53.##Hanif N, Wu H, Xu P, Li Y, Bibi A, Zulfiqar A, et al. Proteomic changes to the updated discovery of engineered insulin and its analogs: pros and cons. Curr Issues Mol Biol 2022 Feb 11;44(2):867-88.##Rodríguez-Sarmiento DL, León-Vargas F, García-Jaramillo M. Artificial pancreas systems: experiences from concept to commercialisation. Expert Rev Med Devices 2022 Nov;19(11):877-94.##Jarosinski MA, Dhayalan B, Rege N, Chatterjee D, Weiss MA. ‘Smart’ insulin-delivery technologies and intrinsic glucose-responsive insulin analogues. Diabetologia 2021 May;64(5):1016-29.##Madani S, Amanzadi M, Aghayan HR, Setudeh A, Rezaei N, Rouhifard M, et al. Investigating the safety and efficacy of hematopoietic and mesenchymal stem cell transplantation for treatment of T1DM: a systematic review and meta-analysis. Syst Rev 2022 May 2;11(1):82.##Khazaei M, Khazaei F, Niromand E, Ghanbari E. Tissue engineering approaches and generation of insulin-producing cells to treat type 1 diabetes. J Drug Target 2023 Jan;31(1):14-31.##Amer LD, Mahoney MJ, Bryant SJ. Tissue engineering approaches to cell-based type 1 diabetes therapy. Tissue Eng Part B Rev 2014 Oct 1;20(5):455-67.##Ashraf MT, Ahmed Rizvi SH, Kashif MAB, Shakeel Khan MK, Ahmed SH, Asghar MS. Efficacy of anti-CD3 monoclonal antibodies in delaying the progression of recent-onset type 1 diabetes mellitus: A systematic review, meta-analyses and meta-regression. Diabetes Obes Metab 2023 Nov;25(11):3377-89.##Zhang S, Deng F, Chen J, Chen F, Wu Z, Li L, et al. Fecal microbiota transplantation treatment of autoimmune-mediated type 1 diabetes: A systematic review. Front Cell Infect Microbiol 2022;12:1075201.##Akhondzadeh S. Hippocampal synaptic plasticity and cognition. J Clin Pharm Ther 1999;24(4):241-8.##Akhondzadeh S. The 5-HT hypothesis of schizophrenia. IDrugs 2001;4(3):295-300.##Akhondzadeh S, Ahmadi-Abhari SA, Assadi SM, Shabestari OL, Kashani AR, Farzanehgan ZM. Double-blind randomized controlled trial of baclofen vs. clonidine in the treatment of opiates withdrawal. J Clin Pharm Ther 2000; 25(5):347-53.##Kashani L, Eslatmanesh S, Saedi N, Niroomand N, Ebrahimi M, Hosseinian M, et al. Comparison of saffron versus fluoxetine in treatment of mild to moderate postpartum depression: a double-blind, randomized clinical trial. Pharmacopsychiatry 2017;50(2):64-8.##

Factor VIII as a Novel Biomarker for Diagnosis, Prognosis, and Therapy Prediction in Human Cancer and Other Disorders 2 2 Coagulation factor VIII (FVIII) is an essential cofactor in the coagulation cascade, encoded by the F8 gene on the long arm of chromosome X (Xq28). FVIII is normally circulated in complex with Von Willebrand factor (VWF) and has relevant emerging extracoagulative functions. Dysregulation of FVIII is associated with tumor progression, and could be used as a novel biomarker for tumor screening and monitoring. In breast cancer, bladder cancer, colorectal carcinoma, esophageal carcinoma, hepatocellular carcinoma and lung cancer, F8 is regarded as an oncogene. In coronary heart disease, hemophilia A and liver disease, F8 dysregulation has been recognized as a potential biomarker for disease diagnosis and prognosis. However, the basis of these differential expression levels remains to be understood. In this review, which is a mixture of literature review and bioinformatics analysis we described the biological functions and characteristics of FVIII, and also its expression level in non-malignant disorders and various cancers. 68 80 Sheyda Khalilian Student Research Committee, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical SciencesUSERN Office, Shahid Beheshti University of Medical Sciences Student Research Committee, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical SciencesUSERN Office, Shahid Beheshti University of Medical Sciences IranIranIran Zahra Mohajer Student Research Committee, School of Medicine, Shahid Beheshti University of Medical SciencesUSERN Office, Shahid Beheshti University of Medical Sciences Student Research Committee, School of Medicine, Shahid Beheshti University of Medical SciencesUSERN Office, Shahid Beheshti University of Medical Sciences IranIran Soudeh Ghafouri-Fard BiomarkersCancerFactor VIIIPrognosis 60569.pdf Khalilian S, Motovali-Bashi M, Rezaie H. Factor VIII: Perspectives on immunogenicity and tolerogenic strategies for Hemophilia A patients. Int J Mol Cell Med 2020;9(1):33-50.##Rezaei H, Motovali-Bashi M, Khalilian S. Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools. Iran J Biotechnol 2021 Apr;19(2):e2700.##Rezaei H, Motovali-Bashi M, Khalilian S. MicroRNA prediction in the FVIII gene locus: A step towards hemophilia A control. Gene Cell Tissue 2020;7:e103096.##Abdulqader AMR, Mohammed AI, Rachid S, Ghoraishizadeh P, Mahmood SN. Identification of the intron 22 and intron 1 inversions of the factor VIII gene in Iraqi Kurdish patients with hemophilia A. Clin Appl Thromb Hemost 2020 Jan-Dec;26:1076029619888293.##Nasirnejad Sola F, Morovvati S, Sabetghadam Moghadam M, Entezari M. Mutation detection and inhibitor risk in Iranian patients with Hemophilia A: Six novel mutations. Clin Case Rep 2020 Dec;8(12):2976-85.##Jalali-Qomi S, Motovali-Bashi M, Rezaei H, Khalilian S. 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The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor 2 2 Background: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and proliferate which gives them healing properties in various tissues. Aluminium chloride (AlCl3) is a chemical compound with harmful effects on health; oxidative stress caused by Aluminium has been reported previously. Crocin, a major component of Crocus sativus (saffron), has antioxidant properties and has shown therapeutic potential. Researchers have been looking for ways to reduce the harmful effects of AlCl3. Methods: To investigate whether crocin can reduce AlCl3 cytotoxicity, rat Bone Marrow Mesenchymal Stem Cells (BM-MSCs) were isolated, cultured and divided into four experimental groups. The first group was the control, which was untreated cells. The second and third groups were treated with crocin (50, 100, 250, 500 µM) and AlCl3 (20, 25, 30 mM) for 24 hr. The fourth group was pre-treated with crocin (250, 500 µM) for 24 hr and then treated with AlCl3 (20 mM) overnight. Cytotoxicity was assessed using the MTT assay. Mineralization was evaluated by alizarin red staining. Sox-2 and E-cadherin expression were measured using real-time PCR. Results: The results showed that AlCl3 caused cytotoxicity on BM-MSCs and decreased the mRNA expression of Sox-2 and E-cadherin, which are important for the maintenance of self-renewal and proliferation of BM-MSCs. In contrast, crocin protected the self-renewal characteristic of BM-MSCs by increasing Sox-2 expression and also preserved the proliferative effects on BM-MSCs by upregulating E-cadherin expression (***p≤0.001). Conclusion: Overall, the study suggests that crocin can protect BM-MSCs from AlCl3-induced cytotoxicity by upregulate Sox-2 expression and E-cadherin expression. This suggests that crocin may be a potential therapeutic agent for the treatment of AlCl3-induced toxicity. 81 87 Elaheh Amini Zahra Baharvand Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences Iran Azadeh Niknejad Department of Cellular & Molecular Biology, Faculty of Biological Sciences, Kharazmi University Department of Cellular & Molecular Biology, Faculty of Biological Sciences, Kharazmi University Iran Yasaman Tabari Department of Cell and Molecular Biology, University of Science and Culture, Royan Institute, ACECR Iran Sahel Shemshadi Department of Cell and Developmental Biology, Julius-Maximilians-University Department of Cell and Developmental Biology, Julius-Maximilians-University Germany Aluminum chlorideAnimalsCadherinsCrocusOxidative stressRats 60570.pdf Bellavia D, Dimarco E, Costa V, Carina V, Luca AD, Raimondi L, et al. 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Food Chem Toxicol 2018 Jun;116(Pt B):307-14.##Yoon DS, Choi Y, Jang Y, Lee M, Choi WJ, Kim SH, et al. SIRT1 directly regulates SOX2 to maintain self‐renewal and multipotency in bone marrow‐derived mesenchymal stem cells. Stem Cells 2014 Dec;32(12):3219-31.##Rudiansyah M, El-Sehrawy AA, Ahmad I, Mergia Terefe E, Abdelbasset WK, Bokov DO, et al. Osteoporosis treatment by mesenchymal stromal/stem cells and their exosomes: Emphasis on signaling pathways and mechanisms. Life Sci 2022 Oct 1:306:120717.##Gall TM, Frampton AE. Gene of the month: E-cadherin (CDH1). J Clin Pathol 2013 Nov;66(11):928-32.##Soncin F, Ward CM. The function of e-cadherin in stem cell pluripotency and self-renewal. Genes (Basel) 2011 Feb 25;2(1):229-59.##Conacci-Sorrel, M, Simcha I, Ben-Yedidia T, Blechman J, Savagner P, Ben-Ze'ev A. Autoregulation of E-cadherin expression by cadherin–cadherin interactions: the roles of β-catenin signaling, Slug, and MAPK. 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The Protective Effect of N-acetylcysteine against Deltamethrin-Induced Hepatotoxicity in Mice 2 2 Background: Exposure to pesticides is of concern to public health officials worldwide. Deltamethrin is a synthetic pyrethroid pesticide which is widely used in agriculture and veterinary medicine. Deltamethrin poisoning is always one of the concerns in medical centers due to the deltamethrin induced hepatotoxicity. This study evaluated the hepatoprotective effects of N-acetylcysteine (NAC) against deltamethrin induced hepatotoxicity in mice Methods: A total of 40 BALB/c male mice were randomly divided into four groups; the first group was used as a control (0.5 ml normal saline); Groups 2-4 were treated with NAC [160 mg/kg Body Weight (BW)], deltamethrin (50 mg/kg BW), and NAC plus deltamethrin. At 1 and 24 hr after treatment, the animals were sacrificed and blood and liver samples were obtained for analysis and the liver/body ration, hepatic enzymes as Aspartate aminotransferase (AST), Alanine Transaminase (ALT), Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), Glutathione (GSH) content and Reactive Oxygen Species (ROS) level were measured. For comparison between more than two experimental groups, one-way ANOVA following Tukey test was used by SPSS software. Results: The deltamethrin significantly increased AST, ALT, ALP, and the level of ROS level at the end of 1 and 24 hr after treatment; while the LDH level and GSH content were decreased. Mice in the deltamethrin treated group had a higher liver/body weight ratio than in other treated groups after 24 hr. On the other hand, NAC in combination with deltamethrin significantly reduced the activities of AST, ALT, ALP, and increased GSH levels. Conclusion: This study demonstrated that NAC has a hepatoprotective role against deltamethrin-induced toxicity. 88 94 Ali Ameri Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Iran Alireza Rahmati Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Iran Shadi Soroushfar Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences Iran Mehdi Lalehzari Trauma Research Center, Hormozgan University of Medical Sciences Trauma Research Center, Hormozgan University of Medical Sciences Iran Tahereh Dehghani Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences Iran Hamed Haghi-Aminjan Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences Iran Jebreil Shamseddin Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences Iran Mahmoud Omidi Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hormozgan University of Medical Sciences Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hormozgan University of Medical Sciences Iran DeltamethrinHepatoprotectionLiverN-acetylcysteinePesticides 60571.pdf Cunha FDS, Sousa NDC, Santos RFB, Meneses JO, do Couto MVS, de Almeida FTC, et al. Deltamethrin-induced nuclear erythrocyte alteration and damage to the gills and liver of Colossoma macropomum. Environ Sci Pollut Res Int 2018;25(15):15102-10.##Hołyńska-Iwan I, Szewczyk-Golec K. Pyrethroids: how they affect human and animal health? Medicina (Kaunas, Lithuania) 2020;56(11).##Parlato F, Buendia Palacios D, Adão-Serrano M, Gonçalves F, Carreiro C, Gouveia JL. A suicide attempt: deltamethrin intoxication. Eur J Case Rep Intern Med 2022;9(9):003573.##Hooven LA, Sherman KA, Butcher S, Giebultowicz JM. Does the clock make the poison? Circadian variation in response to pesticides. PloS One 2009;4(7):e6469.##Azmi MA, Naqvi SN, Azmi MA, Aslam M. Effect of pesticide residues on health and different enzyme levels in the blood of farm workers from Gadap (rural area) Karachi-Pakistan. Chemosphere 2006;64(10):1739-44.##Maroni M, Fanetti AC, Metruccio F. Risk assessment and management of occupational exposure to pesticides in agriculture. La Med Lav 2006;97(2):430-7.##Abhilash PC, Singh N. Pesticide use and application: an Indian scenario. J Hazard Mater 2009;165(1-3):1-12.##Mallick P, Moreau M, Song G, Efremenko AY, Pendse SN, Creek MR, et al. Development and application of a life-stage physiologically based pharmacokinetic (PBPK) model to the assessment of internal dose of pyrethroids in humans. Toxicol Sci 2020;173(1):86-99.##Rogliani P, Matera MG, Page C, Puxeddu E, Cazzola M, Calzetta L. Efficacy and safety profile of mucolytic/antioxidant agents in chronic obstructive pulmonary disease: a comparative analysis across erdosteine, carbocysteine, and N-acetylcysteine. Respir Rese 2019;20(1):104.##Zhang Q, Ju Y, Ma Y, Wang T. N-acetylcysteine improves oxidative stress and inflammatory response in patients with community acquired pneumonia: A randomized controlled trial. Medicine 2018;97(45):e13087.##Patriarca S, Furfaro AL, Domenicotti C, Odetti P, Cottalasso D, Marinari UM, et al. Supplementation with N-acetylcysteine and taurine failed to restore glutathione content in liver of streptozotocin-induced diabetics rats but protected from oxidative stress. Biochim Biophysica Acta 2005;1741(1-2):48-54.##de Andrade KQ, Moura FA, dos Santos JM, de Araújo OR, de Farias Santos JC, Goulart MO. Oxidative stress and inflammation in hepatic diseases: therapeutic possibilities of N-acetylcysteine. Int J Mol Sci 2015;16(12):30269-308.##Otrubová O, Turecký L, Uličná O, Janega P, Luha J, Muchová J. Therapeutic effects of N-acetyl-L-cysteine on liver damage induced by long-term CCl4 administration. Gen Physiol Biophys 2018;37(1):23-31.##Oda SS, El-Maddawy Z. Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Exp Toxicol Pathol 2012;64(7-8):813-9.##Takasaki I, Oose K, Otaki Y, Ihara D, Fukuchi M, Tabuchi A, et al. Type II pyrethroid deltamethrin produces antidepressant-like effects in mice. 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A Simple High Yield Technique for Isolation of Wharton's Jelly-derived Mesenchymal Stem Cell 2 2 Background: The isolation of Mesenchymal Stem Cells (MSCs) from various tissues is possible, with the umbilical cord emerging as a competitive alternative to bone marrow. In order to fulfill the demands of cell therapy, it is essential to generate stem cells on a clinical scale while minimizing time, cost, and contamination. Here is a simple and effective protocol for isolating MSC from Wharton's Jelly (WJ-MSC) using the explant method with various supplements. Methods: Utilizing the explant method, small fragments of Wharton's jelly from the human umbilical cord were cultured in a flask. The multipotency of the isolated cells, were confirmed by their differentiation ability to osteocyte and adipocyte. Additionally, the immunophenotyping of WJ-MSCs showed positive expression of CD73, CD90, and CD105, while remaining negative for hematopoietic markers CD34 and CD45, meeting the criteria for WJ-MSC identification. Following that, to evaluate cells' proliferative capacity, various supplements, including basic Fibroblast Growth Factor (bFGF), Non-Essential amino acids (NEA), and L-Glutamine (L-Gln) were added to either alpha-Minimal Essential Medium (α-MEM) or Dulbecco's Modified Eagle's Medium-F12 (DMEM-F12), as the basic culture media. Results: WJ-MSCs isolated by the explant method were removed from the tissue after seven days and transferred to the culture medium. These cells differentiated into adipocyte and osteocyte lineages, expressing CD73, CD90, and CD105 positively and CD34 and CD45 negatively. The results revealed that addition of bFGF to α-MEM or DMEM-F12 media significantly increased the proliferation of MSCs when compared to the control group. However, there were no significant differences observed when NEA or L-Gln were added. Conclusion: Although bFGF considerably enhances cell proliferation, our study demonstrates that MSCs can grow and expand when properly prepared Wharton's jelly tissues of the human umbilical cord. 95 103 Bahare Niknam Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Iran Arezou Azizsoltani Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences Iran Neda Heidari Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Iran Samaneh Tokhanbigli Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Iran Helia Alavifard Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Iran Mahsa Haji Valili Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Iran Davar Amani Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences Iran Hamid Asadzadeh Aghdaei Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences Iran Seyed Mahmoud Hashemi Kaveh Baghaei Fibroblast growth factor 2Mesenchymal stem cellsUmbilical cord 60572.pdf Friedenstein AJ, Chailakhyan RK, Latsinik NV, Panasyuk AF, Keiliss-Borok IV. 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Phase II multicenter, randomized, double-blind controlled study of efficacy and safety of umbilical cord-derived mesenchymal stromal cells in the prophylaxis of chronic graft-versus-host disease after HLA-haploidentical stem-cell transplantation. J Clin Oncol 2016 Aug 20;34(24):2843-50.##Friedenstein AJ, Gorskaja J, Kulagina N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 1976;4(5):267-74.##Fraser JK, Wulur I, Alfonso Z, Hedrick MH. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 2006;24(4):150-4.##Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, et al. Collection, cryopreservation, and characterization of human dental pulp–derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods 2008;14(2):149-56.##Cao C, Dong Y, Dong Y. [Study on culture and in vitro osteogenesis of blood-derived human mesenchymal stem cells]. 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Simple Determination of Bosentan in Plasma Samples by Reversed-Phase High-Performance Liquid Chromatography 2 2 Background: In order to measure the plasma levels of Losartan and Bosentan, a sensitive Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) technique was developed. Methods: To compare bioavailability, the Area Under the Curve (AUC), peak plasma concentration (Cmax), and time to Cmax (Tmax) were employed. The standard curve (150-2400 ng/ml) was linear (R2=0.999), relative errors were between 2.4 to 10.05% and the coefficient of variation (CV%) ranged from 1.52 to 10.88. A single dosage (test and reference) was used for the in vivo investigation, which involved 16 healthy individuals. Results: The AUC0-48, AUC0-, Cmax, and Tmax of the test and reference had no statistically significant differences. The Cmax and 95% confidence intervals of the ratio of Cmax of the two formulations were 0.93-0.96 and 97.6-135%, respectively. Conclusion: Therefore, it was established that generic Bosentan was equivalent to Bosentan from Actelion and that both medications could be regarded as equally effective in clinical settings. The blood level of Bosentan could be measured using this straightforward procedure in all hospital laboratories. 104 110 Zahra Khalighi Department of Internal Medicine, School of Medicine, Shahid Mustafa Khomeini Hospital, Ilam University of Medical SciencesBiotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical Sciences Department of Internal Medicine, School of Medicine, Shahid Mustafa Khomeini Hospital, Ilam University of Medical SciencesBiotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical Sciences IranIran Hori Ghaneialvar Biotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences Biotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences IranIran Armin Soltani Department of Internal Medicine, School of Medicine, Shahid Mustafa Khomeini Hospital, Ilam University of Medical Sciences Department of Internal Medicine, School of Medicine, Shahid Mustafa Khomeini Hospital, Ilam University of Medical Sciences Iran Ali Khorshidi Department of Epidemiology and Biostatistics, School of Medicine, Ilam University of Medical Science Department of Epidemiology and Biostatistics, School of Medicine, Ilam University of Medical Science Iran Elahe Karimi Ardeshir Moayeri Department of Anatomy, School of Medicine, Ilam University of Medical Sciences Department of Anatomy, School of Medicine, Ilam University of Medical Sciences Iran Naser Abbasi Biotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical SciencesDepartment of Pharmacology, School of Medicine, Ilam University of Medical Sciences Biotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical SciencesDepartment of Pharmacology, School of Medicine, Ilam University of Medical Sciences IranIran Masoumeh Tahmasebi Department of Emergency Medicine, School of Medicine, Ilam University of Medical Sciences Department of Emergency Medicine, School of Medicine, Ilam University of Medical Sciences Iran Ali Aidy BioequivalenceBosentanHigh performance liquid chromatographyLosartan 60573.pdf Rubin LJ, Badesch DB, Barst RJ, Galie N, Black CM, Keogh A, et al. 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Poultry Gastrointestinal-derived Lactic Acid Bacteria (pGIT-d-LAB) Inhibit Multiple Antibiotics Resistance Bacterial and Fungal Pathogens 2 2 Background: To develop a probiotic formulation for poultry feed, a few poultry gastrointestinal derived lactic acid bacteria (pGIT-d-LAB) were isolated from chicken intestinal specimens and in vitro experiment was performed to evaluate their efficacy as potential probiotic candidate. Methods: A total of 6 strains of LAB: Lactobacillus brevis (L. brevis), Lactobacillus acidophilus (L. acidophilus), Lactobacillus casei (L. casei), Pediococci spp., Lactobacillus fermentum (L. fermentum) and Lactobacillus plantarum (L. plantarum) were isolated and cultured for collection of Cell Free Supernatant (CFS). CFS collected was tested against pathogenic bacterial isolated from chicken feces as well as prevalent fungal pathogens, utilizing agar-well diffusion techniques. A preliminary investigation into the susceptibility of the pathogens to diverse antibiotics and antifungal drugs was conducted. Bacterial pathogens exhibiting resistance to a minimum of three classes of antibiotics were subsequently identified for pGIT-d-LAB CFS screening. Results: The observed results revealed that the CFS derived from the isolates exhibited varying degrees of growth inhibition against different pathogens. Among the tested pGIT-d-LAB isolates, L. acidophilus demonstrated the most prominent zone of inhibition, measuring 18 mm against Klebsiella pneumoniae ZTAC 1233. Notably, Citrobacter diversus ZTAC 1255 showed resistance to all tested pGIT-d-LAB. Quantification of the metabolites produced was performed, and peak production levels was determined. L. acidophilus produced the highest amount of lactic acid (1.789g/l), Pediococci spp. produced the highest amount of diacetyl and H202 (1.918g/l) (0.0025g/l) at 48 hr peak values respectively. Conclusion: The test isolates are potential probiotic candidates for controlling pathogens in poultry. 111 119 Bolanle Adeniyi Abimbola Adesuyi Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Nigeria Funmilola Ayeni Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Nigeria Temitope Ogunbanwo Department of Microbiology, Faculty of Science, University of Ibadan Department of Microbiology, Faculty of Science, University of Ibadan Nigeria Taiwo Agidigbi Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan Nigeria Antibacterial agentsChickensLactic acidPoultryProbiotics 60574.pdf Wang Y, Wu J, Lv M, Shao Z, Hungwe M, Wang J, et al. 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Investigating the Effects of HMGB1 Overexpression on Colorectal Cancer Cell Migration via Oncolytic Herpes simplex Virus Type 1 (oHSV-1) 2 2 Background: Colorectal Cancer (CRC) represents a significant global health challenge, and its progression, resistance to therapy, and metastasis are strongly influenced by the tumor microenvironment, including factors like hypoxia. This study explores the impact of High Mobility Group Box 1 (HMGB1) overexpression on CRC cell migration, while identifying potential genes associated with this process. Methods: To explore this, we developed oncolytic virotherapy, resulting in HSV-HMGB1, an oncolytic Herpes simplex virus that expresses HMGB1. HMGB1 is known its role in cancer progression, particularly in the context of cancer cell migration. Results: Contrary to expectations, our scratch assays indicated that HSV-HMGB1 did not significantly induce migration in CRC cells, suggesting that HMGB1 might not directly contribute to this process. Employing microarray analysis, we investigated gene expression changes linked to CRC cell migration, leading to construction of a Protein-Protein Interaction (PPI) network. This network revealed the presence of hub proteins, including as NDRG1, LGALS1, and ANGPTL4, which are recognized for their roles in cancer cell migration. The differential expression of these genes under hypoxic conditions was further validated using quantitative RT-PCR, aligning with the findings from our microarray data.  Conclusion: Our findings emphasize the complex regulation of CRC cell migration, and provides valuable insights into potential molecular mechanisms and pathways. These findings have implications for further research into cancer progression and the development of therapeutic strategies. 120 129 Sara Shayan Department of Molecular Virology, Pasteur Institute of Iran Iran Arash Arashkia Department of Molecular Virology, Pasteur Institute of Iran Iran Golnaz Bahramali Department of Hepatitis and AIDS and Blood Borne Diseases, Pasteur Institute of Iran Iran Kayhan Azadmanesh Colorectal neoplasmsGalectin 1HMGB1 proteinOncolytic virotherapySimplex virusTumor microenvironment 60575.pdf Rawla P, Sunkara T, Barsouk A. Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors. Prz Gastroenterol 2019;14(2):89-103.##Siegel RL, Wagle NS, Cercek A, Smith RA, Jemal A. Colorectal cancer statistics, 2023. CA Cancer J Clin 2023;73(3):233-54.##Li Y, Zhao L, Li XF. Hypoxia and the tumor microenvironment. Technol Cancer Res Treat 2021;20:15330338211036304.##Roy S, Kumaravel S, Sharma A, Duran CL, Bayless KJ, Chakraborty S. Hypoxic tumor microenvironment: Implications for cancer therapy. Exp Biol Med (Maywood) 2020;245(13):1073-86.##Mi Y, Mu L, Huang K, Hu Y, Yan C, Zhao H, et al. Hypoxic colorectal cancer cells promote metastasis of normoxic cancer cells depending on IL-8/p65 signaling pathway. Cell Death Dis 2020;11(7):610.##Höckel M, Vaupel P. Tumor hypoxia: definitions and current clinical, biologic, and molecular aspects. J Natl Cancer Inst 2001;93(4):266-76.##Muz B, de la Puente P, Azab F, Azab AK. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia (Auckl) 2015;3:83-92.##Kaufman HL, Kohlhapp FJ, Zloza A. Oncolytic viruses: a new class of immunotherapy drugs. Nat Rev Drug Discov 2015;14(9):642-62.##Friedman GK, Haas MC, Kelly VM, Markert JM, Gillespie GY, Cassady KA. Hypoxia moderates γ(1)34.5-deleted Herpes simplex virus oncolytic activity in human glioma xenoline primary cultures. 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CYP21A2 Gene Analysis in Southern Iranian CAH Patients and a Brief Review of the Mutation Spectrum 2 2 Background: CYP21A2 gene mutations are responsible for more than 95% of Congenital Adrenal Hyperplasia (CAH) disorders with autosomal recessive inheritance. Most of these pathogenic mutations originate from the CYP21A1P, a neighboring pseudogene with 98% homology, due to unequal crossing over or gene conversion events. Mutation identification of the gene could be beneficial for accurate diagnosis and outcome prediction. Methods: Twelve unrelated patients with CAH diagnosis were recruited for genetic counseling. To ensure distinct amplification of the CYP21A2 gene rather than its pseudogene, the complete sequence of the gene was amplified through two overlapping fragments by specific primers. The entire sequences were screened by direct Sanger sequencing using new sequencing primers. Results: Only two pathogenic point mutations were identified. The c.293-13C>G, also known as In2G, and the c.955C>T mutations were found in 37.5 and 33.3% of alleles, respectively. One patient showed homozygous gene deletion. We also reviewed recent reports on CYP21A2 gene mutations in Iran. Conclusion: Evaluating the ethnicity-specific gene mutation data is significant for populations with diverse ethnic groups including the Iranian population. Although several common mutations have been reported as causative mutations among CAH patients, identifying only two common point mutations in Fars province would help prioritize exon sequencing and reduce the cost and time of genotyping. 130 135 Danial Zangene Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences Iran Ali Moravvej Neonatal Research Center, Shiraz University of Medical Sciences Neonatal Research Center, Shiraz University of Medical Sciences Iran Homa Ilkhanipoor Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Iran Anis Amirhakimi Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Iran Zhila Afshar Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Department of Pediatric Endocrinology, School of Medicine, Shiraz University of Medical Sciences Iran Mona Entezam Adrenal hyperplasiaCongenitalGenotyping techniquesMutation 60576.pdf Carvalho B, Marques CJ, Santos-Silva R, Fontoura M, Carvalho D, Carvalho F. 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