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2008-2835
2008-4625
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>2010
>April-June
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23407609
Conjugation of R-Phycoerythrin to a Polyclonal Antibody and F (ab')2 Fragment of a Polyclonal Antibody by Two Different Methods
R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. Conjugation of antibodies to R-PE facilitates multiple fluorescent staining methods. In the present study polyclonal antibodies and polyclonal F(ab')2 fragment antibodies were conjugated to R-PE by two different methods. The efficiency of the methods was evaluated using Immunocytochemistry (ICC) and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). In the first conjugation method, PE was attached to SMCC linker followed by conjugation of antibody to PE-SMCC. In the second method, SH groups were added onto R-PE molecule, while the antibody was attached to SPDP linker. Then, the antibody-SPDP molecule was conjugated to R-PE. Our results showed that the two conjugation methods did not have any abrogative effects on the antibody binding activity.
Antibody, Conjugation, Immunocytochemistry, Phycoerythrin
87
92
https://www.ajmb.org/En/Article.aspx?id=34
https://www.ajmb.org/PDF/En/FullText/34.pdf
JafarMahmoudianMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran114
MahmoodJeddi-TehraniImmune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Institute, Stockholm, Sweden15
HodjattallahRabbaniImmune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Institute, Stockholm , Sweden24
Ahmad RezaMahmoudiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran33
Mohammad MehdiAkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran13
Amir-HassanZarnaniNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran7
LeilaBalaei GoliMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran119
MahdokhtBabaeiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran120
RoyaGhodsMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran8