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Editorial
I have recently sent out a letter to many of our colleagues and friends in the Iranian scientific community informing them of the recent addition of AJMB to two of the world’s largest databases, namely: Scopus and Embase. In case the spectrum of the coverage by these databases are not known to the AJMB readers in Iran, I have decided to provide you with some information about these two databases obtained from the official company website. Scopus (launched in November 2004) is a database of abstracts and citations and covers nearly 18,000 titles from more than 5,000 international publishers, including coverage of 16,500 peer-reviewed journals in the scientific, technical, medical and social sciences as well as fields in arts and humanities. It is owned by Elsevier and searches in Scopus incorporate searches of scientific web pages (435 Million), patent databases (23 Million) from 5 patent offices (US Patent and Trademark Office, European Patent Office, Japan Patent Office, World Intellectual Property Organization and UK Intellectual Property Office), article-in-press from over 3000 journals and full coverage of Medline titles.
Embase or the Excerpta Medica Database is a biomedical and pharmacological database produced also by Elsevier and contains over 11 million records from 1947 to the present date. Each record is fully indexed and covers over 5,000 biomedical journals from 70 countries and is available online through a number of database vendors. Embase has a holding of more than 2,000 biomedical titles that are not offered by the Medline. Embase delivers comprehensive, authoritative, and reliable coverage of the most relevant biomedical literature.
Now that AJMB has been recognized as a journal deserving to be included in these databases, it is up to the Iranian scientists to support the AJMB’s mission of publishing high quality articles in the field of Medical Biotechnology from Iran and worldwide. I continue to look forward to receiving your high quality articles and would appreciate if you could inform and encourage your colleagues to submit their articles to AJMB and enjoy its international exposure among thousands of other medical scientific journals in the world.
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https://www.ajmb.org/En/Article.aspx?id=158
https://www.ajmb.org/PDF/En/FullText/158.pdf
AliM. ArdekaniReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran2
en
23407796
Production of Monoclonal Antibody against Human Nestin
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.
Antibodies, Blotting, Immunohistochemistry, Monoclonal, Nestin, Peptides, Western
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https://www.ajmb.org/En/Article.aspx?id=32
https://www.ajmb.org/PDF/En/FullText/32.pdf
RezaHadaviMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran111
Amir-HassanZarnaniNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran7
NegahAhmadvandMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran112
Ahmad RezaMahmoudiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran33
Ali AhmadBayatMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran12
JafarMahmoudianMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran114
Mohammad RezaSadeghiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran40
HalehSoltanghoraeeReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran39
Mohammad MehdiAkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran13
MajidTarahomiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran38
MahmoodJeddi-TehraniMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran15
HodjattallahRabbaniImmune and Gene Therapy Lab, CCK, Department of Oncology-Pathology, Karolinska University Hospital Solna, Karolinska Institutet , Stockholm, Sweden24
en
23407651
Angiotensin II Differentially Induces Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Production and Disturbs MMP/TIMP Balance
Angiotensin II, the main component of the renin-angiotensin system, is associated with cardiovascular diseases such as hypertension, vascular remodeling and inflammation. Remodeling process results from dysregulation of Matrix Metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). MMPs are considered as important target genes for angiotensin II. The aim of this study was to determine the effects of angiotensin II on MMP-9 and TIMP-1 production and MMP/TIMP balance in a monocytic cell type. Human monocytic U-937 cells were cultured and treated with 100 nM angiotensin II. Supernatants were analyzed for MMP-9 and TIMP-1 using ELISA and zymography methods. Real-time PCR was utilized to evaluate relative MMP-9 and TIMP-1 genes expression following treatments. Cytotoxicity potentials of treatments were determined by assaying lactate dehydrogenase leakage from the cells. Stimulation of the monocytic cells with angiotensin II significantly increased MMP-9 and TIMP-1 secretion as measured by ELISA (p<0.05). It also augmented gelatinolytic activity of MMP-9 in the conditioned media as much as 49% (p<0.05). Incubation of the cells with angiotensin II for 12 hr increased MMP-9 and TIMP-1 gene expression 2.7 and 1.8 folds, respectively (p<0.05). Angiotensin II treatments did not establish significant cytotoxic effects. In summary, our data provide further evidences that monocytic MMP-9 is a major effector of angiotensin II. It is induced more efficiently than TIMP-1 by angiotensin II that leads to MMP/TIMP imbalance. Our data also reveal the pivotal participation of these cells in pathological cardiovascular remodeling mediated by angiotensin II.
Angiotensin II, Matrix metalloproteinase 9, Monocytic cell, Tissue inhibitor of metalloproteinase-1
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https://www.ajmb.org/En/Article.aspx?id=33
https://www.ajmb.org/PDF/En/FullText/33.pdf
HamidYaghootiDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran116
MohsenFiroozraiSchool of Medicine, Iran University of Medical Sciences, Tehran, Iran, Tehran, Iran117
SoudabehFallahSchool of Medicine, Iran University of Medical Sciences, Tehran, Iran118
Mohammad RezaKhorramizadehDepartment of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran61
en
23407609
Conjugation of R-Phycoerythrin to a Polyclonal Antibody and F (ab')2 Fragment of a Polyclonal Antibody by Two Different Methods
R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. Conjugation of antibodies to R-PE facilitates multiple fluorescent staining methods. In the present study polyclonal antibodies and polyclonal F(ab')2 fragment antibodies were conjugated to R-PE by two different methods. The efficiency of the methods was evaluated using Immunocytochemistry (ICC) and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). In the first conjugation method, PE was attached to SMCC linker followed by conjugation of antibody to PE-SMCC. In the second method, SH groups were added onto R-PE molecule, while the antibody was attached to SPDP linker. Then, the antibody-SPDP molecule was conjugated to R-PE. Our results showed that the two conjugation methods did not have any abrogative effects on the antibody binding activity.
Antibody, Conjugation, Immunocytochemistry, Phycoerythrin
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https://www.ajmb.org/En/Article.aspx?id=34
https://www.ajmb.org/PDF/En/FullText/34.pdf
JafarMahmoudianMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran114
MahmoodJeddi-TehraniImmune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Institute, Stockholm, Sweden15
HodjattallahRabbaniImmune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Institute, Stockholm , Sweden24
Ahmad RezaMahmoudiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran33
Mohammad MehdiAkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran13
Amir-HassanZarnaniNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran7
LeilaBalaei GoliMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran119
MahdokhtBabaeiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran120
RoyaGhodsMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran8
en
23407751
An Investigation into the Antifungal Property of Fabaceae using Bioinformatics Tools
Chemodiversity in plants provides sources of great value which might be helpful for finding new leads in drug discovery programs. Fabaceae as the third largest family of flowering plants was chosen to investigate its possible antifungal activity. In order to increase the effectiveness of the result, molecular similarity methods and chemical data were used. Twelve plants were selected from Fabaceae and collected from the North and South of Iran. Percolation method with 80% ethanol was used for extraction of collected plants. Antifungal activities of these extracts were determined using broth microdilution method against Candida albicans (C. albicans) ATCC 10231, Aspergillus fumigatus (A. fumigatus) AF 293 and Asperigillus niger (A. niger) ATCC 16404. Extracts with promising activity were screened for toxicity with larvae of Artemia salina (brine shrimp). Dalbergia sissoo, Lathyrus pratensis, Oreophysa microphyalla, Astragalus stepporum, Ebenus stellata, Sophora alopecuroides, Ammodendron persicum and Taverniera cuneifolia showed activity against at least one of the microorganisms used in this study. According to the results of our experiment, the extracts of these plants can be used for further investigation in therapeutic research.
Antifungal Research, Bioinformatics, Fabaceae, Plant extracts
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https://www.ajmb.org/En/Article.aspx?id=35
https://www.ajmb.org/PDF/En/FullText/35.pdf
ZahraArabiFaculty of Biological Science, Shahid Beheshti University, Tehran, Iran121
SoroushSardariDrug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute, Tehran, Iran54
en
23407688
Anti-Arthritic Activity of Premna serratifolia Linn., Wood against Adjuvant Induced Arthritis
Adjuvant induced arthritis is a chronic crippling, skeleton-muscular disorder having nearest approximation to human rheumatoid arthritis for which there is currently no medicine available effecting a permanent cure. Even modern drugs used for the amelioration of the symptoms, offer only temporary relief and also produce severe side effects. In the indigenous system of medicine, wood of Premna serratifolia Linn., is reported to be useful in the treatment of arthritis. It is a large shrub, distributed throughout Asia, used against a wide variety of diseases. However, no systematic study has been reported regarding its anti-arthritic activity. This work was aimed at the scientific validation of the ethno-pharmacological claim about its anti-arthritic property. In the present study, anti-arthritic activity of ethanol extract of Premna serratifolia Linn., wood is done by Freund's adjuvant induced arthritis model. Loss in body weight during arthritis condition was corrected on treatment with ethanol extract and standard drug, indomethacin. Biochemical parameters such as hemoglobin content, total WBC, RBC, erythrocyte and sedimentation rate were also estimated. The ethanol extract at the dose of 300 mg/kg body weight inhibited the rat paw edema by 68.32% which is comparable with standard drug indomethacin 74.87% inhibition of rat paw edema after 21 days. The results of the current investigation concluded, ethanol extract of Premna serratifolia Linn., wood possess a significant anti-arthritic activity against adjuvant induced arthritis and justifying its therapeutic role in arthritic condition. The observed anti-arthritic activity may be due to the presence of phytoconstituents such as irridiod glycosides, alkaloids, phenolic compounds and flavonoids.
Arthritis, Freund’s adjuvant, Indomethacin
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https://www.ajmb.org/En/Article.aspx?id=36
https://www.ajmb.org/PDF/En/FullText/36.pdf
RekhaRajendranDepartment of Pharmacognosy and Phytochemistry, Mohamed Sathak A. J. College of Pharmacy, Tamil Nadu, India122
EkambaramKrishnakumarDepartment of Pharmaceutical Biotechnology, Balaji Institute of Pharmaceutical Sciences, Warangal, AP, India123
en
23407866
The Effect of Biopsy During Precompacted Morula Stage on Post Vitrification Development of Blastocyst Derived Bovine Embryos
Improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. The aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different precompacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. The in vitro produced bovine embryos were subjected to biopsy at days 2, 3, and 4 post-insemination with different cell numbers (4 to 16-cells). Embryo cell biopsy was carried out in a 100 ?l drop of H-SOF following pronase drilling by aspiration of one blastomere. The biopsied embryos were then cultured in SOFaaBSA co-cultured with oviduct cells-monolayer until blastocyst formation. The blastocysts were cryopreserved at room tempera-ture after exposure of equilibration (glycerol 1.4 M for 5 min and then glycerol 1.4 M and ethylene glycol 3.6 M for 5 min) and vitrification solutions (3.4 M glycerol and 4.6 M ethylene glycol). The blastocysts were loaded into the center of 0.25 ml straws separated by air bubbles from 2 columns of sucrose 0.5 M and plunged immediately into liquid nitrogen. There was no significant difference in cryosurvivability of vitrified-warmed blastocysts derived form biopsied embryos at different pre-compacted morula stages. The quality of biopsy derived blastocysts was identical to that of non-biopsy derived ones in terms of post vitrifcation survival and hatching rates. In conclusion there was no preference between different times of embryo biopsy at precompacted morula stages in term of cryosurvivability of biopsy derived bovine blastocysts.
Bovine, Cryopreservation, Embryo Research, Fertilization in Vitro
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https://www.ajmb.org/En/Article.aspx?id=37
https://www.ajmb.org/PDF/En/FullText/37.pdf
AbolfazlShiraziReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran82
SaraBorjianReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran87
EbrahimAhmadiResearch Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran84
HassanNazariResearch Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran85
BanafshehHeidariReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran86