The Protein Subcellular Mislocalization in Human Cancers

en 32153731 The Protein Subcellular Mislocalization in Human Cancers Several sets of proteins exist in eukaryotic cells wherein accurately localized in appropriate cellular locations containing plasma membrane, cytoplasm, nucleus and different membrane-enclosed organelles. Each protein is involved in distinct biochemical processes within the normal cell; therefore, the correct subcellular localization of protein is vital; as it provides the physiological context for protein function. However, protein mislocalization described as changing the appropriate subcellular localization of protein, was reported as a key feature of many proteins in a variety of human cancers 1. Mislocalization has important implications in alteration of activation condition, biological function and interaction network of a protein. Particularly,  the aberrant localization of tumor-suppressor proteins and proto-oncoproteins can alter their functions, respectively in either suppressing or supporting the cancer initiation in normal cells whereby cancer development, metastasis and drug resistance are increased 2.  The mechanisms by which subcellular mislocalization is arising in cancer are various and deeply reviewed by Wang and Li 2. They can be described as modification of signals directing proteins into a particular location, dysregulation of sorter and transporter machinery, Endoplasmic Reticulum (ER) retention of misfolded proteins, aberrant endocytosis and vesicular trafficking, dysregulation of signal transduction and protein post-translational modification and so on 2. The aberrant subcellular position of proteins in cancer tissues has been investigated broadly by antibody-based strategies including Immunohistochemistry (IHC). The major localization of sortilin on cell surface of ovarian carcinoma tissues rather than the main resident in ER-Golgi compartment of normal tissues was detected using IHC technique 3. The new method of Dissociable Antibody Microarray (DAMA) combining the power of IHC staining with protein microarray, provides the facility of high-throughput detection of protein subcellular localization 4. The property of malignant cells in differently subcellular localization of proteins might be recruited as an intelligent strategy for detection of malignant but not normal cells in clinical diagnostic and prognostic applications 5.  For instance, fibromodulin, a type of proteoglycan resides in extracellular matrix, has been irregularly located on cell surface of Chronic Lymphocytic Leukemia (CLL) cells but not in normal samples by which a new CLL diagnostic biomarker appropriate for cell surface flow cytometric detection  might be suggested 6. Moreover, directly targeting of locations in where proteins are accumulated in cancer cells plus selecting the structural dissimilarities of proteins by biologic weapons help us to specifically eradicate malignant but not normal cells. In conclusion, the subscellular mislocalization of proteins is a frequent event in cancers defined as an accessory approach for proliferation, survival and invasion of tumors. However, targeting and trapping proteins in specific cellular compartments has been conceptualized as a promising approach for diagnostic, prognostic and therapeutic clinical use. 1 1 https://www.ajmb.org/En/Article.aspx?id=20411 https://www.ajmb.org/PDF/En/FullText/20411.pdf FatemehGhaemimaneshMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran764

en 32153732 AS-LAMP: A New and Alternative Method for Genotyping In recent decades, different methods have been introduced for the genotyping of Single Nucleotide Polymorphisms (SNPs) and mutations in nucleic acid sequences. These methods have several applications ranging from agriculture to medicine. The Loop-mediated isothermal amplification (LAMP) method was first introduced by Notomi et al. Since then, different methods derived from LAMP have been extensively applied in detecting pathogens. The LAMP method is an isothermal technique that amplifies the target DNA segment using four different primers that have been uniquely designed for recognizing six distinct zones on the objective gene; the process of reaction continues at a constant temperature via a strand displacement reaction. Amplifying and detecting the targeted zone can be accomplished in one stage. Although the LAMP method is mostly used for pathogen detection, several studies have used this method for genotyping. The present article reviewed various studies that used the LAMP method for SNP detection. The outcomes indicated that the LAMP technique could be a reliable and alternative technique for genotyping. Further studies are recommended to use this approach for genotyping. AS-LAMP, Genotyping, LAMP, Single nucleotide polymorphisms 2 8 https://www.ajmb.org/En/Article.aspx?id=20412 https://www.ajmb.org/PDF/En/FullText/20412.pdf PooriaGillDepartment of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran21547ArashHadian-AmreeStudent Research Committee, Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran21503

en 32153733 Evaluation of Immune Response and Protection Induced by V-ATPase Subunit F as DNA Vaccine Against Leishmania tropica (LCED Syrian 01) After Detection and Sequencing Background: Leishmaniasis is one of the major emerging health problems worldwide and Leishmania tropica (L. tropica) is most prevalent in the Middle East due to conflict and environmental factors, and there is no effective prevention strategy available until now. An effective vaccine has not been developed to date. DNA vaccines are considered a promising approach to protect against this infection. In this study, since vacuolar (H+)-ATPase (V-ATPase) enzyme has an essential role in the life cycle of eukaryotes, V-ATPase subunit F gene has been chosen to design DNA vaccine and evaluate its immunogenicity in BALB\c mice.  Methods: Genomic DNA was isolated from promastigote culture, synthesized complementary DNA (cDNA) after standardization of Polymerase Chain Reaction (PCR) conditions. The V-ATPase subunit F gene was placed into plasmid PCI. Then, recombinant plasmids were transformed into competent cells. Cloning was confirmed by PCR, restriction enzyme assays, and finally, DNA sequence analysis, after making miniprep from positive colonies and finally the gene was sequenced. BALB/c mice were immunized subcutaneously three times at an interval of two weeks with designed vaccine. BALB\c mice were challenged with 106 promastigotes of L. tropica 7 days post-immunization. IL-12, IFN-γ and IL-4 were quantified by RT-qPCR.  Results: The present study proved the existence of subunit F gene in Syrian strain of L. tropica (LCED Syrian 01) promastigotes genome. Its expression was also proved in these parasites and the gene length was 414 bp.  Conclusion: This study showed that vaccination of BALB\c mice with this gene induced partial protection against Leishmania by reduction of lesion size by 41.9% and parasite burden reduction by 3-log in the dLNs when compared with control group. IFN-γ\IL-4 was 1.6 after challenge test, so the immune response consisted of both Th1 and Th2. BALB\c mice, DNA vaccine, Leishmania tropica, Parasite load, RT-PCR, V-ATPase subunit F 9 16 https://www.ajmb.org/En/Article.aspx?id=10397 https://www.ajmb.org/PDF/En/FullText/10397.pdf AmiraOrabiDepartment of Biochemistry and Parasitology, Faculty of Pharmacy, Damascus University, Damascus, Syria21527MohammadMaaroufDepartment of Biochemistry and Parasitology, Faculty of Pharmacy, Damascus University, Damascus, Syria21528MustafaAlammoriDepartment of Biochemistry and Parasitology, Faculty of Pharmacy, Damascus University, Damascus, Syria21529

en 32153734 Optimization of In vitro Expansion and Activation of Human Natural Killer Cells against a Breast Cancer Cell Line Background: Regarding to the increase of cancer deaths in recent years and disability of common therapies to eradicate cancers, as well as expansion of Natural Killer (NK) cell therapy, it seems so vital to find new useful therapies against cancers. Breast cancer is the second main cause of cancer death among women. As it is impossible for a majority of patients to receive NK cell therapy, an attempt was made to establish a low-cost and efficient method for expanding and activating NK cells against breast cancer cell line (MCF7). Methods: NK cells were isolated from Peripheral Blood Mononuclear Cells (PBMCs) applying either MACS based NK cell enrichment kit or antibodies and complement as cytotoxic method. Then, the NK cells were cultured in Stem Cell Growth Medium (SCGM) with feeder layer (irradiated PBMCs) along with PHA or OKT3. IL-2, IL-15 and IL-21 were used to expand NK cells and finally their cytotoxic activity was investigated by flow cytometry. Results: Highly pure NK cells were obtained and no significant difference between the two isolation methods was found. Using IL-2 plus IL-15, the number of NK cells increased up to100 fold after 16 days. No significant effect was observed after IL-21 treatment. Conclusion: Our data indicated that cytotoxicity method can be considered a low-cost alternative for NK cell isolation kits. It seems that culturing NK cells for 14 days in either PHA or OKT3 supplemented SCGM medium would be more effective than culturing for 16 days in the presence of IL-21. Interleukins, Immunotherapy, Natural killer cells 17 23 https://www.ajmb.org/En/Article.aspx?id=10400 https://www.ajmb.org/PDF/En/FullText/10400.pdf FarzanehPeighambarzadehDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran21534AnahitaNajafalizadehDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran21535NafisehEsmaeilDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran21536AbbasRezaeiDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran21537FarzanehAshrafiHematology Division, Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran21538MazdakGanjalikhani-HakemiDepartment of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran438

en 32153735 Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells Background: Placenta-specific 1 (PLAC1), as a new Cancer/Testis Antigen (CTA), is frequently expressed in a variety of cancers and localized to cytoplasm and plasma membrane. Surface expression of cancer target antigens is of great importance that enables antibody-mediated cancer immunotherapy. The aim of the current study was to express the intact human PLAC1 protein on plasma membrane of a eukaryotic cell as a model for future anti-PLAC1-based cancer immunotherapy. Methods: In the first approach, entire human PLAC1 gene including its own Signal Peptide (SP) was cloned into pIRES2-EGFP and LeGO-iG2 vectors and expressed in CHO-K1 cells. In the second approach, cytosolic and Signal-Anchor (SA) sequence of Transferrin Receptor Protein 1 (TFR1) were fused to extracellular portion of PLAC1 and expressed as above. Expression of PLAC1 was then assessed using Reverse Transcription Polymerase Chain Reaction (RT-PCR), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) and Flow Cytometry (FC). Results: The first approach resulted in the expression of PLAC1 in submembranous but not in the surface of transfected CHO-K1 cells. Using the chimeric human PLAC1 construct, the same intracellular expression pattern was observed. Conclusion: These results indicated that there are some yet unknown PLAC1 localization signals employed by cancer cells for surface expression of PLAC1. Eukaryotic cells, PLAC1 protein expression, Protein transport 24 31 https://www.ajmb.org/En/Article.aspx?id=10402 https://www.ajmb.org/PDF/En/FullText/10402.pdf JafarMahmoudianNanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran21539MahboobehNazariMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran11474RoyaGhodsDepartment of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran8MahmoodJeddi-TehraniMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran15Seyed NaserOstadDepartment of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran153Mohammad HosseinGhahremaniDepartment of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran692SedighehVafaeiReproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran21541Mohammad MehdiAmiriDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran21542Amir-HassanZarnaniImmunology Research Center (IRC), Iran University of Medical Sciences (IUMS), Tehran, Iran7

en 32153736 Effect of Poly-Histidine Tag Position toward Inhibition Activity of Secretory Leukocyte Protease Inhibitor as Candidate for Material Wound Healing Background: The Secretory Leukocyte Protease Inhibitors (SLPI) has many biological functions including anti-bacterial, anti-fungal, anti-viral, anti-inflammatory, and immuno-modulatory. Previous studies have shown that gene-encoding human SLPI have successfully been expressed in Escherichia coli (E. coli) with a C-terminal polyhistidine tag (His-tag). The aim of this research was to investigate the inhibition activity of N-terminal His-tag position (NSLPI) and C-terminal His-tag position (CSLPI). We hypothesized that a His-tag close to an active site SLPI domain may interfere with the inhibition activity of SLPIs.  Methods: A NSLPI and CSLPI were constructed with polymerase chain reaction (PCR) amplification. The PCR products were then ligated into pET-30a plasmid and transformed into E. coli TOP10. Recombinant plasmids were verified by using restriction analysis and nucleotide sequence analysis. pET-NSLPI and pET-CSLPI were then subcloned in E. coli BL21(DE3) for its expression. The SLPI protein was expressed using Isopropyl β-D-1-thiogalactopyranoside induction (IPTG). The inhibition effect of both SLPI against Porcine Pancreatic Elastase (PPE) enzyme was tested using the N-succinyil-alanyl-L-alanyl-L-prolyl-L-phenylalanyl-4-nitroanalide (NPN) substrate.  Results: The SLPI gene was successfully cloned and expressed in E. coli BL21. Fusion proteins of NSLPI and CSLPI were generated with His-tag in the N-terminal and C-terminal position, respectively. The inhibition effect of NSLPI and CSLPI on PPE indicated that both SLPI were active. The inhibition activity of NSLPI was 66.7%, higher than CSLPI by 44.4%.  Conclusion: The His-tag position on the C-terminal of SLPI reduced the inhibition activity of SLPI. Escherichia coli (E. coli), Gene expression, Poly histidine, Secretory leukocyte protease inhibitor 32 36 https://www.ajmb.org/En/Article.aspx?id=20413 https://www.ajmb.org/PDF/En/FullText/20413.pdf EllyMunadzirohDepartment of Dental Material and Technology, Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia31550Evi UlfaDepartment of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia31551AmaliahLabiqahDepartment of Health Analysis, Stikes Kesetiakawanan Sosial Indonesia, Jakarta, Indonesia31552OneAsmaraniProteomic Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia31553Ni NyomanPuspaningsihProteomic Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia31554

en 32153737 A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments Background: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient SOEing PCR assay was developed for simultaneous splicing of multiple DNA fragments and induction of site-directed mutagenesis in a single tube. Methods: The method was adapted for splicing human beta-globin UTRs to OCT4, SOX2, KLF4, C-MYC, LIN28A, and destabilized GFP for the construction of chimeric DNA fragments for in vitro transcription. In addition, the native Kozak sequence of beta-globin (K1) was replaced by the strongest Kozak sequence (K2) using site-directed mutagenesis to enhance the expression of target genes. Results: ChimericGFPd2/K1, GFPd2/K2, OCT4, and KLF4 were created by the optimized conventional SOEing PCR. The single tube method was able to create the chimeric SOX2, C-MYC, and LIN28A in high quality and quantity in comparison with the conventional SOEing PCR. Moreover, using single tube SOEing PCR, the reaction time and materials that are required in the conventional SOEing PCR were significantly reduced. Fluorescent microscopy and flow cytometry examinations indicated highly efficient translation of K2 sequence in comparison with the K1sequence. Conclusion: Single tube SOEing PCR is a valuable method to construct more multiple fragments with high yield. The method can successfully be applied for construction of various kinds of complex chimeric genes. Beta-globins, Mutagenesis, Polymerase chain reaction , Site-directed, Untranslated regions 37 43 https://www.ajmb.org/En/Article.aspx?id=20415 https://www.ajmb.org/PDF/En/FullText/20415.pdf FarzanehZarghampoor Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran31555AbbasBehzad-Behbahani899NegarAzarpira672Saeed RezaKhatami Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran31556MaryamFanianTransplant Research Centre, Shiraz University of Medical Science, Shiraz, Iran31557MahdokhtHossein-AghdaieTransplant Research Centre, Shiraz University of Medical Science, Shiraz, Iran31558Gholam RezaRafiei DehbidiDiagnostic Laboratory Sciences and Technology Research Centre, Faculty of Paramedical Sciences, Shiraz University of Medical Science, Shiraz, Iran988

en 32153738 HR9: An Important Cell Penetrating Peptide for Delivery of HCV NS3 DNA into HEK-293T Cells Background: The delivery of exogenous genes into cells for functional expression is required for development of DNA vaccine and gene therapy in medicine and pharmacology. Cell Penetrating Peptides (CPPs) were considered to mediate gene and drug delivery into living cells. In this study, an attempt was made to evaluate the efficiency of an arginine-rich CPP, HR9, in HCV NS3 gene delivery compared to TurboFect cationic polymer and supercharged +36 GFP into HEK-293T cells. Methods: The recombinant pEGFP-NS3 was constructed and their accuracy was confirmed by digestion and sequencing. Then, the recombinant plasmid was transfected into HEK-293T cells by TurboFect, +36 GFP and HR9 gene delivery systems. The expression of NS3 protein was assessed by fluorescent microscopy, flow cytometry and western blotting. Results: Our data indicated that HR9 peptide was able to form stable complexes with plasmid DNA and increased its delivery into HEK-293T cells in a non-covalent manner. Furthermore, treatment of cells with HR9 and HR9/DNA complexes resulted in a viability of 90-95% indicating this CPP was not cytotoxic. The analysis of zeta potential and size showed the importance of interactions between positively-charged HR9/pEGFP-NS3 complexes and negatively-charged plasma membranes.   Conclusion: The non-toxic HR9 CPP can be considered an effective carrier for delivering plasmid DNA harboring Hepatitis C virus (HCV) gene in therapeutic vaccine design. Cell penetrating peptide, DNA, Gene delivery system, Hepatitic C virus, Vaccines 44 51 https://www.ajmb.org/En/Article.aspx?id=20416 https://www.ajmb.org/PDF/En/FullText/20416.pdf SinaAlizadehDepartment of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran31560ShivaIraniDepartment of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran31561AzamBolhassaniDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran50Seyed MehdiSadatDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran817

en 32153739 Investigation of the Correlation between Androgen Receptor and ZEB1 and its Value in Progression of Gastric Cancer Background: Zinc-finger Enhancer Binding protein (ZEB1) acts as a transcription factor to promote cancer progression through regulating Epithelial to Mesenchymal Transition (EMT). It is well-known that ZEB1 mRNA expression is directly induced by both Estrogen Receptor (ER) and Progesterone Receptor (PR). Moreover, Androgen Receptor (AR) and PR could bind to the same regulatory element. Since it has been shown that AR overexpresses in Gastric Cancer (GC) as a male-predominant tumor, the goal of this study was to evaluate whether AR could regulate ZEB1 expression in GC. Methods: The expression profile of ZEB1 in 60 fresh GC and adjacent non-tumor tissues and 50 normal gastric specimens was assessed by qRT-PCR, and the association of ZEB1 expression with clinicopathological features was investigated. Furthermore, possible correlation between ZEB1 and AR was evaluated to elucidate a novel prognostic marker using Kaplan-Meier method and Cox regression model. Finally, molecular interaction of ZEB1 and AR was assessed using a potent AR antagonist in GC cells. Results: Among GC patients, 70.2% (40/57) overexpressed ZEB1 and 64.91% (37/57) overexpressed AR relative to normal gastric tissues. ZEB1 overexpression was significantly correlated with the AR overexpression in GC patients. Moreover, ZEB1 overexpression was remarkably associated with lower overall survival; however, it was not an independent prognostic factor. Evidence shows that simultaneous evaluation of ZEB1 and AR expression could independently predict survival of GC patients (HR=2.193, p=0.047). Conclusion: These findings have clinical importance suggesting simultaneous evaluation of ZEB1 and AR expression as a potential prognostic marker. Moreover, AR may regulate ZEB1 expression in GC cells proposing a possible promising targeted therapy for GC patients. Androgen receptor, Enzalutamide, Gastric cancer, Prognostic marker, Targeted therapy, ZEB1 52 60 https://www.ajmb.org/En/Article.aspx?id=20419 https://www.ajmb.org/PDF/En/FullText/20419.pdf ShahrzadSoleymani FardHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran92MasoudSotoudehDigestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran31562KioomarsSaliminejadReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran393MansourYazdanbodDepartment of Surgery, Madaen Hospital, Tehran, Iran31563HabibollahMahmoodzadehDepartment of Surgical Oncology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran31564ShaghayeghKouchakiHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran31565MarjanYaghmaeiHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran373Seyed AsadollahMousaviHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran31507RezaMalekzadehDigestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran31568KamranAlimoghaddamHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran31569Seyed HamidollahGhaffariHematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran31570

en 32153740 Evaluation of the Bioactive Potential of Secondary Metabolites Produced by a New Marine Micrococcus Species Isolated from the Persian Gulf Background: In the present work, a newly isolated marine bacterium, Micrococcus sp. MP76, from coastal area of Persian Gulf around Bushehr province, Iran, was identified with the ability to produce bioactive compounds. Methods: The pigment production was optimized by changing carbon and nitrogen sources in bacterial growth media at 28°C and 220 rpm for 5 days. Partial purification of the pigment was carried out using suitable solvents. Results: Maximum pigment extract was achieved (1.4 g/l) when cultured in the medium containing 0.5% (v/v) molasses, 0.5% (w/v) peptone, 1% (w/v) sea salt, 0.01% (w/v) potassium phosphate, and 0.05% (w/v) yeast extract, pH=7.0. Antibacterial effect assessment of the extract against pathogenic bacteria revealed the MIC values in the range of 4.2-7.5 mg/ml depending on different pathogens. The pigment extracted from medium supplemented by molasses and ammonium sulfate had 81% radical scavenging activity, and its IC50 value was 0.28 mg/ml. Conclusion: The newly isolated strain of Micrococcus genus from the Persian Gulf revealed a valuable source to access worth medicinal ingredients when cultured under optimized conditions. Antibacterial effect, Antioxidant activity, Bioactive compounds, Marine bacteria, Persian Gulf 61 65 https://www.ajmb.org/En/Article.aspx?id=20420 https://www.ajmb.org/PDF/En/FullText/20420.pdf Hamid RezaKarbalaei-HeidariInstitute of Biotechnology, Shiraz University, Shiraz, Iran31518MozhdehPartovifarMolecular Biotechnology Lab., Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran31571MinaMemarpoor-YazdiMolecular Biotechnology Lab., Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran31572

en 32153741 Importance of Argan Oil in Human Health According to the Dosage of Antioxidants in the Algerian Argan Fruits (Argania spinosa) Antioxidants, Argan oil, Health, Human, Morocco 66 66 https://www.ajmb.org/En/Article.aspx?id=20421 https://www.ajmb.org/PDF/En/FullText/20421.pdf ZohraBenaoufResearch Laboratory in Geo Environment and Spatial Development LGEDE, University of Mustapha Stambouli, Mascara, Algeria31573ImenBenbahiLaboratory of Plant Ecology and Environment, Faculty of Biological Sciences, USTHB University, Bab Zouar, Algiers, Algeria31574OussamaDjorfLaboratory of Biochemistry, Faculty of Chemistry, USTHB University, Bab Zouar, Algiers, Algeria31575ZahiraSouidiResearch Laboratory on Biological Systems and Geomancy (L.R.S.B.G), University of Mustapha Stambouli, Mascara, Algeria31576RedaKechairiLaboratory of Plant Ecology and Environment, Faculty of Biological Sciences, Telemcen University, Telemcen, Algeria31577