en
2008-2835
2008-4625
276
5669
11181
gregorian
>2019
>October-December
11
4
online
1
fulltext
en
31908738
A Metabolomic Study to Identify Potential Tissue Biomarkers for Indomethacin-Induced Gastric Ulcer in Rats
<p>Background: Gastric Ulcer (GU) is the most prevalent gastrointestinal disorder in-duced by various factors and Non-Steroid Anti-Inflammatory Drugs (NSAIDs) as one of the most common reasons. Due to the absence of appropriate molecular markers for GU, the aim of this study was to utilize a metabolomics approach in order to find potential metabolite markers for the disease. <br />
Methods: Stomach tissue samples from indomethacin-treated rats and normal con-trols were used to perform a 1H-NMR metabolomics study. The altered metabolites were identified using random forest multivariate analysis.<br />
Results: ROC curves showed that the random forest model had a good predictive performance with AUC of 1 for the test and 0.708 for the training sets. Seventeen differentially expressed metabolites were found between GU and normal tissue sample. These metabolites included trimethylamine, betaine, carnitine, methionine, acetylcho line, choline, N,N-Dimethylglycine, cis-aconitate, tryptophan, spermidine, acetylcar-nitine, creatinine, pantothenate, taurine, isoleucine, glucose and kynurenine.<br />
Conclusion: The results of the study demonstrated that metabolomics approach could serve as a viable method to find potential markers for GU. Surely, further studies are needed for the validation of the results.</p>
Gastric ulcer, Indomethacin , Metabolomics, Nuclear magnetic resonance
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https://www.ajmb.org/En/Article.aspx?id=10390
https://www.ajmb.org/PDF/En/FullText/10390.pdf
ReyhanehFarrokhi-YektaProteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran21504
NasrinAmiri-DashatanStudent Research Committee, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran21505
MehdiKoushkiDepartment of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran21506
MasoomehDadpayDepartment of Pathology, AJA University of Medical Sciences, Tehran, Iran21507
FatemehGoshadrouDepartment of Basic Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran1225