en
2008-2835
2008-4625
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11181
gregorian
>2019
>January-March
11
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30800248
Expression and Activity of Platelet Endothelial Nitric Oxide Synthase are Decreased in Patients with Coronary Thrombosis and Stenosis
<p>Background: Nitric Oxide (NO) which is synthesized by endothelial Nitric Oxide Synthase (eNOS) in both vascular tissues and platelets plays an important role as a protective mediator in the cardiovascular system. It modulates blood pressure, vasodilation and thrombosis. In this regard, eNOS activity and gene expression in platelets and NO levels in patients’ plasmas with Coronary Thrombosis (CT) and stenosis diseases were determined. <br />
Methods: Blood samples were collected from 60 subjects that where divided into three equal groups [without coronary artery disease, with CT and less than 70% Coronary Stenosis (CS)]. NO concentration in plasma was measured by the Griess reagent system. The eNOS activity was assessed based on a fluorimetric detection system in platelets and the gene expression was quantified by the real time-reverse transcription-polymerase chain reaction. <br />
Results: There was a significant decrease in the amount of NO concentration in the plasma of subjects with CT (0.53±0.09 <em>µM</em>, p<0.01) and CS (1.31±0.11 <em>µM</em>, p<0.01) compared to the control group (2.6±0.10 <em>µM</em>). The activity levels of eNOS enzyme were significantly lower in patients’ platelets with CT (0.68±0.013 <em>UF/mn</em>, p<0.01) and CS (0.85±0.017 <em>UF/mn</em>, p<0.01) than the control cases (1.29±0.019 <em>UF/mn</em>). These data were consistent with the reduction of the expression levels of eNOS in patients with CT (75 folds) and CS (4 folds) lower than the control cases.<br />
Conclusion: Patients with CT and CS possessed reduced eNOS activity and gene expression in their platelets. Decreased plasma concentration of NO in these patients confirmed the potential significance of the diagnostic and prognostic value of NO in the subjects’ plasma with vascular disease risk.</p>
Cardiovascular diseases, eNOS, Nitric oxide, Platelets, Real time PCR, Thrombosis
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https://www.ajmb.org/En/Article.aspx?id=10349
https://www.ajmb.org/PDF/En/FullText/10349.pdf
ZahraEmamiDepartment of Biochemistry, Faculty of Medicine, Shiraz University of Medical Sciences, Tehran, Iran11374
AlirezaMesbah NaminDepartment of Biochemistry, Tarbiat Modaress University, Tehran, Iran11375
JavadKojuriDepartment of Cardiology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran11376
FaridehMashayekhi JalaliDepartment of Biochemistry, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran11377
MozhganRastiDepartment of Biochemistry, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran808