en
2008-2835
2008-4625
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>2018
>April-June
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29849986
Clinically Significant Dysregulation of hsa-miR-30d-5p and hsa-let-7b Expression in Patients with Surgically Resected Non-Small Cell Lung Cancer
<p>Background: The cyclin E2 (CYCE2) is an important regulator in the progression and development of NSCLC, and its ectopic expression promoted the proliferation, invasion, and migration in several tumors, including Non-Small Cell Lung Cancer (NSCLC). However, the upregulation of CYCE2 in NSCLC cells suggested that it has a key role in tumorigenicity. In addition, the RAS family proteins as oncoproteins were activated in many major tumor types and its suitability as the therapeutic target in NSCLC was proposed. Considering the crucial role of microRNAs, it was hypothesized that altered expression of <em>hsa-miR-30d-5p</em> and <em>hsa-let-7b</em> might provide a reliable diagnostic tumor marker for diagnosis of NSCLC.<br />
Method: Real-time RT-PCR approach could evaluate the expression alteration of <em>hsa-miR-30d-5p </em>and <em>hsa-let-7b</em> and it was related to the surgically resected tissue of 24 lung cancer patients and 10 non-cancerous patients. The miRNAs expression was associated with clinicopathological features of the patients.<br />
Results: <em>Hsa-miR-30d</em> showed a significant downregulation (p=0.0382) in resected tissue of NSCLC patients compared with control group. Its expression level could differentiate different stages of malignancies from each other. The ROC curve analysis gave it an AUC=0.73 (p=0.037) which was a good score as a reliable biomarker. In contrast, <em>hsa-let-7b</em> was significantly overexpressed in tumor samples (p=0.03). Interestingly, our findings revealed a significant association of <em>hsa-let-7b</em> in adenocarcinoma tumors, compared to Squamous Cell Carcinomas (SCC) (p<0.05). Also, analysis of ROC curve of <em>hsa-let-7b</em> (AUC=0.74, p-value=0.042) suggests that it could be as a suitable biomarker for NSCLC.<br />
Conclusion: Together, these results suggest a possible tumor suppressor role for <em>hsa-miR-30d</em> in lung tumor progression and initiation. Moreover, upregulation of <em>hsa-let-7b</em> was associated with the tumor type.</p>
Lung cancer, MicroRNAs, Tumor markers
98
104
https://www.ajmb.org/En/Article.aspx?id=311
https://www.ajmb.org/PDF/En/FullText/311.pdf
Sayed MostafaHosseiniDepartment of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1242
BahramMohammad SoltaniDepartment of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1141
MahmoudTavallaeiHuman Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran1243
Seyed JavadMowlaDepartment of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran248
ElhamTafsiriDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran1244
AbouzarBagheriDepartment of Clinical Biochemistry and Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran1165
Hamid RezaKhorram KhorshidGenetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran42