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2008-2835
2008-4625
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gregorian
>2022
>October-December
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36504567
Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds
<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Biofilm formation helps <em>Pseudomonas </em></span><em><span style="font-size:10.0pt">‎</span></em><em><span style="font-size:10.0pt">aeruginosa</span></em><span style="font-size:10.0pt"> (<em>P. </em></span><em><span style="font-size:10.0pt">‎</span></em><em><span style="font-size:10.0pt">aeruginosa</span></em><span style="font-size:10.0pt">) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropyl-acrylamide</span> <span style="font-size:10.0pt">microgels (PNIPAM) on <em>P. aeruginosa</em>.</span></span></p>
<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> Totally</span><span style="font-size:10.0pt">‎</span><span style="font-size:10.0pt">, </span><span style="font-size:10.0pt">‎</span><span style="font-size:10.0pt">100 <em>P. aeruginosa</em> strains were isolated from chronic wound infections</span><span style="font-size:10.0pt">‎. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, <em>alg</em>D, <em>las</em>R, and <em>PA</em>2714<em> </em>genes were amplified to investigate gene frequencies and expression rates.</span></span></p>
<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results: </span><span style="font-size:10.0pt">Significant decreases were seen in <em>las</em>R expression in EDTA-treated samples</span><span style="font-size:10.0pt">‎</span><span style="font-size:10.0pt">. Significant decreases were observed in expression of <em>alg</em>D and <em>las</em>R treated with xylitol. Decreased </span><span style="font-size:10.0pt">‎expression of <em>PA</em>2714 was seen in samples treated with xylitol with no significance. </span></span></p>
<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> The PNIPAM containing xylitol </span><span style="font-size:10.0pt">‎or EDTA could penetrate biofilms of <em>P. aeruginosa</em> and significantly decrease expression of <em>las</em>R and <em>alg</em>D. This can be a novel strategy in the management of chronic wounds.</span></span></p>
Biofilms, Microgels, Pseudomonas aeruginosa, Real-time PCR
317
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https://www.ajmb.org/En/Article.aspx?id=60520
https://www.ajmb.org/PDF/En/FullText/60520.pdf
AkramEtemadinia Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran91934
AmirSeyfoori Department of Biomaterials and Tissue Engineering, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran91935
AbbasRahimi Foroushani Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran91936
RaminMazaheri Nezhad FardFood Microbiology Research Center, Tehran University of Medical Science, Tehran, Iran81819
RonakBakhtiari 91938