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2008-2835
2008-4625
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gregorian
>2009
>April-June
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23407719
Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)
A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. Taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial T vector, suggests that it may be one of the best T vectors for cloning of PCR products.
Cloning, PCR products, pGEM-5zf(+), T vector
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https://www.ajmb.org/En/Article.aspx?id=6
https://www.ajmb.org/PDF/En/FullText/6.pdf
YaofengZhaoDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden19
ZhancaiLiuDepartment of Physics, Chemistry and Biology, Jiaozuo Teachers College, Jiaozuo, 454001 , Henan, P. R. China20
ShuyangYuDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden21
SichengWenDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden22
LennartHammarstromDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden23
HodjattallahRabbaniDepartment of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, Iran24