en <p><span style="font-size:11pt">Background: Inhibition of angiogenesis is an attractive approach in cancer therapy. Both curcumin and silver nanoparticles (AgNPs) have demonstrated anti-angiogenic properties; however, the the poor water solubility of Curcumin and the side effects of AgNPs adversely affect their activity. </span></p> <p><span style="font-size:11pt">Methods: In this study, AgNPs coated with curcumin (Cur-AgNPs), was used to improve aqueous-phase solubility of curcumin and decrease the side effects of AgNPs. After-wards, treatment with curcumin enhanced the anti-angiogenic activity of Cur-AgNPs. The nanoparticles were synthesized as both reducing and stabilizing agents. Evaluation of anti-angiogenesis was assessed in vitro using Human Umbilical Vein Endothelial Cells (HUVECs) and in vivo through the Chorioallantoic Membrane (CAM) assay. Da-ta were analyzed by one-way ANOVA with Tukey&rsquo;s multiple comparison test. </span></p> <p><span style="font-size:11pt">Results: Synthesized Cur-AgNPs have an average diameter of 39 nm, with spherical shapes and an absorbance peak at 450 nm in the UV-visible spectrum. Cur-AgNPs showed a negative zeta potential. EDAX and FTIR confirmed the conjugation of cur-cumin with AgNPs. In vitro anti-angiogenesis assays demonstrated that Cur-AgNPs re-duced the viability of HUVECs, an Inhibitory Concentration (IC50) value of 13 &micro;g/ml. DAPI and acridine orange/propidium iodide staining revealed a significant increase in apoptotic cells following treatment with Cur-AgNPs. The expression of Matrix Metallo-proteinase 9 (MMP-9) and Cyclooxygenase-2 (COX-2) was also inhibited in treated cells. In vivo anti-angiogenesis assays using the CAM model showed significant de-crease in the number, length and hemoglobin content of CAM blood vessels. </span></p> <p><span style="font-size:11pt">Conclusion: Curcumin conjugated with AgNPs may represent a promising strategy to enhance the therapeutic potential of both AgNPs and curcumin. However, further in-vestigations, particularly regarding safety and biocompatibility of Cur-AgNPs, is needed in this field.</span></p> Angiogenesis, Curcumin, Nanoparticles, Silver 147 156 https://www.ajmb.org/En/Article.aspx?id=70651 https://www.ajmb.org/PDF/En/FullText/70651.pdf TayebeRamezani Farzin 92501MohammadNabiuni Department of Biology, Kharazmi University, Tehran, Iran92502