en <p>Background: Ultraviolet (UV) radiation poses a significant health risk, particularly in high-exposure regions including Indonesia, contributing to more than 1.5 million UV-related Disability-Adjusted Life Years (DALYs) globally due to its involvement in photoaging, skin cancers, and chronic inflammation. This study aimed to evaluate the Curcuma xanthorrhiza extract (CXE) mitigating effects against UV-induced damage in human dermal fibroblasts (BJ cells) by assessing gene expression, protein integrity, DNA damage, and collagen levels. Methods: BJ fibroblasts were irradiated to UV radiation and given CXE at 3.13&ndash;12.5 &micro;g/ml concentrations. TIMP1 and CASP3 gene expression were analyzed via qRT-PCR, while total protein, 8-hydroxy-2&prime;-deoxyguanosine (8-OHdG), and collagen content were measured using ELISA. Results: CXE treatment significantly upregulated TIMP1 and downregulated CASP3 expression in a concentration-dependent manner, with the strongest effects showed at 12.5 &micro;g/ml (p&lt;0.05). At the same concentration, CXE significantly restored total protein levels, reduced 8-OHdG accumulation, and preserved collagen content compared with the UV-induced control (p&lt;0.05). Conclusion: These findings suggest CXE exerts reparative effects against UV-induced photoaging through antioxidant, anti-apoptotic, and Extracellular Matrix (ECM) preserving mechanisms, supporting its potential as a botanical anti-aging therapy.</p> 8-OHdG, CASP3, Collagen, Curcuma, TIMP1 48 54 https://www.ajmb.org/En/Article.aspx?id=70637 https://www.ajmb.org/PDF/En/FullText/70637.pdf WahyuWidowati92419MeilinahHidayatFaculty of Medicine, Maranatha Christian University, Bandung, Indonesia, Bandung, Indonesia92452RitaTjokropranotoFaculty of Medicine, Maranatha Christian University, Bandung, Indonesia92453DwiNur Triharsiwi Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia92454HannaSari Widya KusumaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia92144