en <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> Type 2 diabetes mellitus (T2DM) is accompanied by a significant risk of oxidative stress. While a link between T2DM and G6PD deficiency has been suggested, their interaction is not precisely understood. Furthermore,</span><span style="font-size:10.0pt"> emerging evidence suggests an expression association between <em>G6PD</em> and <em>miR-1, miR-122,</em> and <em>miR-206</em>. Given the antioxidant and anti-inflammatory properties of Curcumin (Cur) and Quercetin (Q), </span><span style="font-size:10.0pt">This study aimed to assess the effects of </span><span style="font-size:10.0pt">curcumin and quercetin </span><span style="font-size:10.0pt">on G6PD expression and its correlation with the mentioned microRNA expression in liver, renal, heart, and muscle in rats with T2DM.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> RT-qPCR was employed to determine <em>miR-1</em>, <em>miR-122</em>, <em>miR-206</em>, and <em>G6PD</em> expression. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> The findings revealed that curcumin and quercetin treatment elevated <em>G6PD</em> gene expression. Also, the treated groups exhibited down-regulation of <em>miR-1</em>, <em>miR-122</em>, and <em>miR-206</em> (p&lt;0.05). Furthermore, there was a significant inverse correlation between <em>G6PD</em> and <em>miR-1</em> in heart, <em>miR-122</em> in all tissues except renal and <em>miR-206</em> expression in skeletal muscle and heart (p&lt;0.05).</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> This study suggests that curcumin and quercetin may prevent the development of T2DM by effectively increasing <em>G6PD</em> expression and reducing <em>miR-1</em>, <em>miR-122</em>, and <em>miR-206</em> expression.</span></span></p> Curcumin, G6PD, miRNA, Quercetin, Type 2 diabetes mellitus 216 224 https://www.ajmb.org/En/Article.aspx?id=70621 https://www.ajmb.org/PDF/En/FullText/70621.pdf MahsimaBagheriInternational Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran92383AmenehKhodarahmi Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran92384FatemehZare MehrjardiDepartment of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran92385AliMoradi92386