en <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> Nanoparticles, as small extracellular vesicles, are considered promising tools in tissue engineering and regenerative medicine. This study aimed to investigate the effects of different processing and culture condition on </span><span style="font-size:10.0pt">the quality and quantity of extracts derived from human Adipose-Mesenchymal Stem Cells (AD-MSCs).</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods</span></strong><strong><span style="font-size:10.0pt">:</span></strong> <strong><span style="font-size:10.0pt">AD-MSCs</span></strong><span style="font-size:10.0pt"> were proliferated in both the experimental and control groups. Nanoparticles were extracted from AD-MSCs-extracts and analyzed using </span><span style="font-size:10.0pt">SEM, TEM, DLS, Zeta potential, FTIR and BCA analyses</span><span style="font-size:10.0pt">. </span><span style="font-size:10.0pt">The morphological characteristics (shape, size, distribution, surface topography, and&nbsp;<strong> </strong>agglomeration/aggregation<strong>), </strong>structural<strong> </strong>appearance (poly-disperse intensity, colloidal particle behavior, surface charge, and stability), chemical properties (functional groups and ionic interactions) and total protein concentration were detected in the extracted nanoparticles.</span> <span style="font-size:10.0pt">Additionally, </span><span style="font-size:10.0pt">the morphological characteristics, apoptosis, </span><span style="font-size:10.0pt">mitochondrial oxidoreductase activity, </span><span style="font-size:10.0pt">and migration potential of AD-MSCs in both groups were evaluated using acridine orange staining, MTT, and scratch assays. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong> <span style="font-size:10.0pt">In the experimental group, 100% of the nanoparticles had a diameter of 112.8&plusmn;25 <em>nm</em>, with the most frequency of 111.4 <em>nm</em>. However, in the control group, 72% of nanoparticles had a diameter of 350.2&plusmn;43.6 <em>nm </em>with the highest frequency of 339.8 <em>nm </em>(</span><span style="font-size:10.0pt">p&le;0.05). </span><span style="font-size:10.0pt">The Z-average, Poly-disperse intensity, and </span><span style="font-size:10.0pt">electrostatic stability</span><span style="font-size:10.0pt"> of nanoparticles in the control and experimental groups were 171.9 <em>nm</em>, 0.727 and </span><span style="font-size:10.0pt">-0.000011 <em>cm²/Vs</em></span><span style="font-size:10.0pt"> <em>vs.</em> 103.7 <em>nm</em>, 0.205 and </span><span style="font-size:10.0pt">0.000481 <em>cm²/Vs,</em></span><span style="font-size:10.0pt"> respectively (</span><span style="font-size:10.0pt">p&le;0.05). </span><span style="font-size:10.0pt">In the experimental group, Zeta potential was -61.8 <em>mV</em>, which is in the range of &zeta; &gt;-30<em>mV</em>. Although, Zeta potential in the control group was -1.5 <em>mV</em>, which is in the range of -30 <em>mV </em>&lt;&zeta; &lt;30 <em>mV</em> (</span><span style="font-size:10.0pt">p&le;0.05</span><span style="font-size:10.0pt">).</span> <span style="font-size:10.0pt">Total protein concentrations in the control and experimental groups were </span><span style="font-size:10.0pt">11 and 41%, respectively (p&le;0.05).</span> </span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion</span></strong><strong><span style="font-size:10.0pt">:</span></strong><span style="font-size:10.0pt"> Nanoparticles derived from AD-MSCs have high therapeutic applications in tissue engineering and regenerative medicine. </span></span></p> Acridine orange, Control groups, Fourier transform infrared, Immunologic factors, Nanoparticles, Obesity, Regenerative medicine, Regenerative medicine, Spectroscopy, Static electricity, Tissue engineering 186 195 https://www.ajmb.org/En/Article.aspx?id=70618 https://www.ajmb.org/PDF/En/FullText/70618.pdf MobinaKarimiDepartment of Biology, SR.C, Islamic Azad University, Tehran, 92372BanafshehHeidari503HaniehJafary Department of Biology, SR.C, Islamic Azad University, Tehran, Iran92373KavoshZandsalimiDepartment of Medical Laser (MLRC), Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran92374