en 40094091 <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> This study addresses the increasing prevalence of type 2 Diabetes Mellitus (T2DM) and the need for alternative, cost-effective medications. The research problem focuses on the need to further study the dose effectiveness of <em>Momordica charantia</em> Extracts (MCE) on T2DM parameters, including Glucagon-Like Peptide 1 (GLP-1), <span style="color:black">Dipeptidyl Peptidase 4</span> (DPP-4), alpha glucosidase, glucosetransporter 5 (GLUT5), and pancreatic tissue histopathology.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> The methodology employed an experimental research design with 30 Wistar rats divided into six groups, each receiving different inductions and doses. Parameters were measured using Elisa, and histological analysis of pancreatic tissue was conducted using HE staining.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> The Kruskal-Wallis test revealed significant differences in each group for the GLP-1 (p=0.003). However, the DPP4 test suggested a lack of significant difference in each group (p=0.192), and the GLUT5 test showed insignificant changes between each group (p=0.119). The ANOVA analysis on alpha-glucosidase revealed no statistically significant differences among the groups (p=0.202). Additionally, a qualitative examination of the histological analysis of pancreatic tissue indicated an improvement in the condition of the pancreatic tissue.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> MCE can increase GLP-1 levels, lower DPP-4, lower alpha-glucosidase, and raise GLUT5. However, there are no significant differences between other groups and the morphology of pancreatic tissue in rat model T2DM at a dose of 300 <em>mg/kg.</em></span></span></p> Dipeptidyl peptidase 4, Glucagon-like peptide 1, Momordica charantia extracts, Rats, Type 2 diabetes mellitus 47 55 https://www.ajmb.org/En/Article.aspx?id=60602 https://www.ajmb.org/PDF/En/FullText/60602.pdf MuhammadFadhol Romdhoni 92303MuchsinDoewes Department of Pharmacology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia92304SoetrisnoSoetrisno Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia92305RatihPuspita Febrinasari Department of Pharmacology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia92306