Therapeutic Effects of Nanochelating-Based Copper Nanoparticles on Burn Wound Healing in Mouse Model

en 40094097 Therapeutic Effects of Nanochelating-Based Copper Nanoparticles on Burn Wound Healing in Mouse Model Background: The aim of the present study was to investigate the potential of Nanochelating-based copper to accelerate the wound healing process and prevent infection in burn wounds. Methods: Six to eight-week- old female BALB/c mice were burned with a 1 cm2 heated copper plate on the left flank and then divided into four treatment groups, treated with C8 (nanochelating-based CuNPs), cold cream (supplementary materials) as a control drug, Silver Sulfadiazine and no treatment, respectively. Skin tissue samples were taken from the mice on days 0, 3, 8, 15 and 24. One piece was fixed in 10% neutral buffered formalin for pathological examination and the others were stored at -80°C until used for pro-inflammatory and growth factor gene expression. Results: The healing process in the group treated with 10 mg/ml C8 was significantly faster, and the survival rate of the mice in this group was significantly higher than in the other groups. The pro-inflammatory genes were expressed and down-regulated earlier in the C8 treated mice. Histopathology confirmed the higher cure rate in the group treated with 10 mg/ml C8 compared to other control groups. Conclusion: C8 has beneficial effects on the healing of burn wounds and the effective dose of this compound should be further investigated. The present study demonstrates the anti-inflammatory properties of nano-chelate-based copper particles' on mouse skin burns. This research opens up new possibilities in dermatology and burn therapy and highlights the potential of copper-based formulations in the treatment of burn injuries. Anti-inflammatory agents, Burns, Copper, Dermatology, Wound healing 2 13 https://www.ajmb.org/En/Article.aspx?id=60597 https://www.ajmb.org/PDF/En/FullText/60597.pdf HosseinRezvan92293Seyed MasoudZolhavariehDepartment of Clinical Sciences, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran92294AlirezaNourianDepartment of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran92295ElhamBayatDepartment of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran92296SomayehKalanakyDepartment of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran92297SaidehFakharzadehDepartment of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran92298PegahKarimiDepartment of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran92299MaryamHafiziDepartment of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran92300SaharHamoon Navard Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran92302Mohammad HassanNazaran92321