en 39606678 <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> Diabetic Foot Ulcer (DFU)</span> <span style="font-size:10.0pt">might be worsened by neuropathy and vascular issues. This condition can cause 14.3% fatality, stressing the need for effective wound healing therapy. Wound healing is a complex biological process, and human Wharton&#39;s Jelly Mesenchymal Stem Cells (hWJMSCs) may help manage DFU treatment issues. This research focuses on utilizing a gel carrier to deliver bioactive substances from Wharton&#39;s Jelly Mesenchymal Stem Cells secretome (hWJ-MSCs-Sec) as a possible treatment for DFU. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> To maintain quality, hWJMSCs-Sec is thoroughly mixed with carbomer gel and freeze-dried. ELISA test is performed to determine the characterization of the gel of hWJMSCs-Sec such as Keratinocyte Growth Factor (KGF), Platelet-Derived Growth Factor (PDGF), Hepatocyte Growth Factor (HGF), Epidermal Growth Factor (EGF), and Heparin-Binding EGF-Like Growth Factor (HB-EGF). The antioxidant activity was also measured with Hydrogen peroxide (H₂O₂), Nitric oxide (NO), and Ferric Reducing Antioxidant Power (FRAP) assay. Proliferation assay was utilized using WST-8 and the wound healing potential was assessed <em>via</em> the migration cell ability of scratched-human skin fibroblast (BJ cells). </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> The freeze-dried hWJ-MSCs-Sec showed higher levels of KGF, HGF, PDGF, EGF, HB-EGF, and the antioxidant activities compared to fresh hWJ-MSCs-Sec. Additionally, the gel of freeze-dried hWJ-MSCs-Sec exhibited higher levels compared to the gel of fresh hWJMSCs-Sec. This was evidenced by faster closure of scratched wounds on BJ cells treated with hWJMSCs-Sec and freeze-dried hWJ-MSCs-Sec gel. </span></span></p> <p><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> The freeze-dried hWJ-MSCs-Sec gel exhibits superior quality compared to the non-freeze-dried hWJ-MSCs-Sec gel. This demonstrates that the freeze-drying procedure can maintain the bioactive chemicals found in hWJMSCs-Sec, potentially enhancing the efficacy of this gel in promoting cell regeneration for wound healing.</span></span></p> Antioxidants, Carbomer gel, Freeze dried secretome gel, hWJ-MSCs, Wound healing 233 243 https://www.ajmb.org/En/Article.aspx?id=60590 https://www.ajmb.org/PDF/En/FullText/60590.pdf WahyuWidowati41601AhmadFariedDr. Hasan Sadikin Hospital, Bandung 40163, Indonesia92239RimontaFebby GunanegaraFaculty of Medicine, Maranatha Christian University, Bandung 40163, Indonesia92240FannyRahardjaFaculty of Medicine, Maranatha Christian University, Bandung 40163, Indonesia92241FadhilahHaifa ZahirohBiomolecular and Biomedical Research Center Bandung, Aretha Medika Utama, Bandung 40163, Indonesia92247AnnisaFirdaus Sutendi Biomolecular and Biomedical Research Center Bandung, Aretha Medika Utama, Bandung 40163, Indonesia92243RizalAzisDepartment of Translational Medical Science, Division of Cancer and Stem Cell, Biodiscovery Institute 3, The University of Nottingham, University Park, United Kingdom NG72RD, Nottingham, United Kingdom NG72RD92245RenandyKristianlie Ekajaya Biology Study Program, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia, Bandung 40163, Indonesia92246