en 39132632 <p><span style="font-size:11pt">Background: Brucellosis vaccines are designed to induce cellular immunity. An effective brucellosis vaccine could induce both cellular and humoral immunity. Serum Bactericidal Assay (SBA) is an important method for determining vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA. </span></p> <p><span style="font-size:11pt">Methods: Extracted Brucella abortus (B. abortus) Lipopolysaccharide (LPS) and Outer Membrane Proteins (OMPs) were injected into rabbits. Group 1 was injected with 25 &micro;g of LPS, Group 2 was injected with 50 &micro;g of OMPs, and Group 3 was injected with 1 ml of combined vaccine, 3 times every 2 weeks. The groups were challenged with B. abortus 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, and each well was streak-cultured into a plate of Brucella agar. A colony count was done for each plate. </span></p> <p><span style="font-size:11pt">Results: Results have shown, the third injection of the combined vaccine had the highest titer of 1/64, and the efficacy of the vaccine was 87.71%. </span></p> <p><span style="font-size:11pt">Conclusion: As a conclusion, the results of this study showed that LPS and OMP&#39;s from B. abortus can provide acceptable immunity.</span></p> Agar, Brucellosis, Cellular, Humoral, Immunity, Lipopolysaccharides, Vaccines 187 192 https://www.ajmb.org/En/Article.aspx?id=60585 https://www.ajmb.org/PDF/En/FullText/60585.pdf BehnamHajizadeh SisakhtDepartment of Bacteriology, Faculty of Veterinary, Islamic Azad University, Science & Research Campus, Tehran, Iran92221MansoorKhalediDepartment of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran92222HamedAfkhamiDepartment of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran92223SaberRouhiResident of Large Animal Internal Medicine, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran92224SaeedSepehrniaDepartment of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran92225VahidehFanaeeDepartment of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran92226HannanehKarimiDepartment of Microbiology, Rasht Islamic Azad University, Rasht, Iran92227YaldaMalekzadegan92228JavadFathi91902MahdiS. Sadati 92229