en 38618510 <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> To develop a probiotic formulation for poultry feed, a few poultry gastrointestinal derived lactic acid bacteria (pGIT-d-LAB) were isolated from chicken intestinal specimens and <em>in vitro</em> experiment was performed to evaluate their efficacy as potential probiotic candidate.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> A total of 6 strains of LAB: <em>L</em><em>actobacillus brevis</em> (<em>L. brevis</em>)<em>,</em> <em>L</em><em>actobacillus acidophilus </em>(<em>L. acidophilus</em>)<em>, Lactobacillus casei </em>(<em>L. casei</em>)<em>, Pediococci </em>spp.<em>, Lactobacillus fermentum </em>(<em>L. fermentum</em>)<em> </em>and<em> L</em><em>actobacillus plantarum </em>(<em>L. plantarum</em>)<em> </em>were isolated and cultured for collection of Cell Free Supernatant (CFS). CFS collected was tested against pathogenic bacterial isolated from chicken feces as well as prevalent fungal pathogens, utilizing agar-well diffusion techniques. A preliminary investigation into the susceptibility of the pathogens to diverse antibiotics and antifungal drugs was conducted. Bacterial pathogens exhibiting resistance to a minimum of three classes of antibiotics were subsequently identified for pGIT-d-LAB CFS screening. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> The observed results revealed that the CFS derived from the isolates exhibited varying degrees of growth inhibition against different pathogens. Among the tested pGIT-d-LAB isolates, <em>L. acidophilus </em>demonstrated the most prominent zone of inhibition, measuring 18 <em>mm</em> against <em>Klebsiella pneumoniae</em> ZTAC 1233. Notably, <em>Citrobacter diversus</em> ZTAC 1255 showed resistance to all tested pGIT-d-LAB. Quantification of the metabolites produced was performed, and peak production levels was determined. <em>L.</em><em> acidophilus </em>produced the highest amount of lactic acid (1.789<em>g/l</em>), <em>Pediococci </em>spp. produced the highest amount of diacetyl and H₂0₂ (1.918<em>g/l</em>) (0.0025<em>g/l</em>) at 48<em> hr</em> peak values respectively. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> The test isolates are potential probiotic candidates for controlling pathogens in poultry. </span></span></p> Antibacterial agents, Chickens, Lactic acid, Poultry, Probiotics 111 119 https://www.ajmb.org/En/Article.aspx?id=60574 https://www.ajmb.org/PDF/En/FullText/60574.pdf BolanleAdeniyi92173AbimbolaAdesuyi Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria92174FunmilolaAyeni Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria92175TemitopeOgunbanwo Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan, Nigeria92176TaiwoAgidigbiDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria92177