Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

en 38605737 Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources Background: Asparagine is an amino acid that can be converted into aspartic acid and ammonia by the enzyme L-asparaginase. Some forms of cancer, such Acute Lymphoblastic Leukaemia (ALL) and Non-Hodgkin Lymphoma (NHL), respond well to this enzyme when employed as a chemotherapeutic drug. The purpose of this research was to find bacteria that can manufacture the enzymes L-asparaginasein marine slattern sediment which can be employed in commercial and industrial scale production. Methods: All of the strains were identified as Bacillus niacini spp. by biochemical and molecular testing. The strain belongs to the Bacillus genus, according to nutritional, biochemical, PCR and 16srRNA sequencing data. Results: According to the findings of this research, Bacillus niacin spp. have the potential to create a substance that is helpful in a variety of medical applications. The results of this study hint to the possibility that bacteria have the ability to produce antimicrobial compounds, which have the potential to be successful in a wide variety of environments. Conclusion: Numerous opportunities may arise for researchers interested in utilizing the medical potential of enzyme-producing bacteria if they are successfully isolated and screened from aquatic and terrestrial habitats. Asparagine, Asparaginase, Bacillus niacini, Polymerase chain reaction 40 48 https://www.ajmb.org/En/Article.aspx?id=60565 https://www.ajmb.org/PDF/En/FullText/60565.pdf Mugip RahamanAbdul Wahab Department of Biotechnology, Dr. M.G.R Educational and Research Institute, Chennai, Tamil Nadu, Iran92131ThirunavukkarasuPalaniyandi Department of Anatomy, Biomedical Research Unit and Laboratory Animal Centre, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, Tamil Nadu, India92132JohnWyson Department of Food Processing Technology, AMET University, Kanathur, Chennai, Tamil Nadu, India92133AshaSivajiDepartment of Biochemistry, DKM College for Women, Vellore-632001, Tamil Nadu, India92134SwarnakalaThamadaMolecular Systematics Laboratory, Zoological Survey of India, Andaman & Nicobar Regional Centre, Port Blair - 744 102, Andaman and Nicobar Islands92135