en <p style="text-align:left"><span style="font-size:12pt"><span style="color:black"><strong>Background:</strong> Cholera is an acute intestinal infection caused by <em>Vibrio cholera </em>(<em>V. cholera</em>). The development of antibodies against specific <em>V. cholerae </em>may have a therapeutic effect. In the present research, we investigated the protective effect of egg yolk Immunoglobulin (IgY), which was produced by immunizing hens with formaldehyde-killed <em>V. cholerae</em> O1 and subsequently the isolated IgY was orally administrated to the <em>V. cholerae</em> O1 infected mice for evaluation of its immunizing capability. </span></span></p> <p style="text-align:left"><span style="font-size:12pt"><span style="color:black"><strong>Methods:</strong> In the current study, hens were immunized three times with<strong> </strong>formaldehyde-killed <em>V. cholerae </em>O1 (1.5&times; 10⁷ <em>CFU/mL</em>) and an equal volume of adjuvant. The IgY was isolated from egg yolk by polyethylene glycol method. The validity and activity of isolated IgY were confirmed with SDS-PAGE and ELISA methods, respectively. Subsequently IgY was orally administered to suckling mice following challenge with <em>V. cholerae</em> O1.<strong> </strong>ELISA results showed high antibody titer in the serum and egg yolk. Also, SDS-PAGE analysis showed successful purification of IgY and anti-<em>V.</em> <em>cholerae</em> IgY prevented the death of mice infected with <em>V.</em> <em>cholerae </em>O1. The anti-<em>V.</em> <em>cholera</em> IgY was administered at 2, 4, 6 hours&rsquo; intervals after 3 hours of inoculation of mice with <em>V.</em> <em>cholerae</em> O1. </span></span></p> <p style="text-align:left"><span style="font-size:12pt"><span style="color:black"><strong>Results:</strong> Results showed that the rate of surviving mice (2 <em>mg/mL</em> of IgY) were 60% after 4 hours and 40% after 6 hours and the rate of surviving mice (5 <em>mg/mL</em> of IgY) was 70% after 4 hours and 60% after 6 hours. </span></span></p> <p style="text-align:left"><span style="font-size:12pt"><span style="color:black"><strong>Conclusion:</strong> The findings suggested the egg yolk-driven IgY as a natural antibacterial protein, could be effective in the prevention and treatment of cholera disease.</span></span></p> Antibodies, Chicken, Immunoglobulin Y, Mice, Vibrio cholerae O1 239 244 https://www.ajmb.org/En/Article.aspx?id=60550 https://www.ajmb.org/PDF/En/FullText/60550.pdf MohammadShoushtariAnatomical Sciences Research Center Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran92073Ali BaratShooshtariKaraj Branch, Islamic Azad University, Karaj, Iran92074SepidehAsadiDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran92075YousofKaramiFaculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran92076MohsenHonariPayame Noor University, Tehran, Iran92077JavadFathi91902MehdiZeinoddini92079Ghorban AliAlizadehPayame Noor University, Tehran, Iran92078