en 36789117 <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><span style="font-size:10.0pt"> Uncontrolled mitosis of cancer cells and resistance cells to chemotherapy drugs are the challenges of prostate cancer. Thalicthuberine causes a mitotic arrest and a reduction of the effects of drug resistance, resulting in cell death. In this study, we applied bioinformatics and computational biology methods to identify functional pathways and side effects in response to Thalicthuberine in prostate cancer patients. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> Microarray data were retrieved from <em><span style="background-color:white">Gene Expression Omnibus</span></em>&nbsp;(GEO), and protein-protein interactions and gene regulatory networks were constructed, using the Cytoscape software. The critical genes and molecular mechanisms in response to Thalicthuberine and its side effects were identified, using the Cytoscape software and WebGestalt server, respectively. Finally, GEPIA2 was used to predict the relationship between critical genes and prostate cancer. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> The&nbsp;<em>POLQ, EGR1, CDKN1A, FOS, MDM2, CDC20, CCNB1,</em> and <em>CCNB2</em> were identified as critical genes in response to this drug. The functional mechanisms of Thalicthuberine include a response to oxygen levels, toxic substances and immobilization stress, cell cycle regulation, regeneration, the p53 signaling pathway, the action of the parathyroid hormone, and the FoxO signaling pathway. Besides, the drug has side effects including muscle cramping, abdominal pains, paresthesia, and metabolic diseases. </span></span></p> <p><span style="font-size:11pt"><span style="font-size:10.0pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> Our model suggested newly predicted crucial genes, molecular mechanisms, and possible side effects of this drug. However, further studies are required.</span></span></span></p> Bioinformatics, Computational biology, Gene expression, Gene regulatory networks, Prostate cancer 53 64 https://www.ajmb.org/En/Article.aspx?id=60527 https://www.ajmb.org/PDF/En/FullText/60527.pdf FatemehSaberi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran91987Zeinab Dehghan Department of Comparative Biomedical Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran91845EffatNoori Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran91989ZahraTaheriDepartment of Biology and Biotechnology, Pavia University, Pavia, Italy91990MarziehSameniCellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran91847HakimehZali91849