en 35633982 <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Non-syndromic cleft lip occurs by the interaction of environmental and genetic factors. The purpose of the current study was to analyze the association of<em> </em></span><span style="font-size:10.0pt">Single Nucleotide Polymorphisms (SNPs) in</span><em><span style="font-size:10.0pt"> IRF6 </span></em><span style="font-size:10.0pt">and NS</span><span style="font-size:10.0pt">CL/P in an Iranian population.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span> <span style="font-size:10.0pt">A group of 105 children with NSCL/P and 185 normal controls were included in the current study. Genotyping of <em>IRF6</em> rs2013162 and rs2235375 was performed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span> <span style="font-size:10.0pt">A substantial association of AA and CA genotypes in rs2013162 with the risk of NSCL/P (AA <em>vs</em>. CC; OR=2.36; 95%CI [1.05-5.29], p=0.004; and CA <em>vs</em>. CC; OR=0.47; 95%CI [0.28-0.79], p=0.018) was found. <span style="background-color:white">However, there were no important associations between A allele and risk of NSCL/P (p=0.980).</span> According to logistic regression analysis results, subjects with GG genotype and G allele in</span><em> </em><span style="font-size:10.0pt">rs2235375</span><span style="font-size:10.0pt"> polymorphism had increased risk of NSCL/P.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> The<em> IRF6 </em>polymorphisms are associated with the susceptibility to NS</span><span style="font-size:10.0pt">CL/P</span><span style="font-size:10.0pt"> in Iranian population.</span></span></p> IRF6, Non-syndromic cleft lip and palate, Orofacial clefts, Polymorphism 181 185 https://www.ajmb.org/En/Article.aspx?id=60502 https://www.ajmb.org/PDF/En/FullText/60502.pdf MasoumehSoleymaniGenetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran91865AsgharEbadifarDentofacial Deformities Research Center, Research Institute of Dental Sciences, Department of Orthodontics, Faculty of Dentistry, Shahid Behehsti University of Medical Sciences, Tehran, Iran891MaryamKhosraviGenetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran91866EmranEsmaeilzadehFetal Health Research Center, Hope Generation Foundation, Tehran, Iran91867Hamid RezaKhorram KhorshidPersonalized Medicine and Genometabolomics Research Center, Hope Generation Foundation, Tehran, Iran42