en 23407719 A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. Taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial T vector, suggests that it may be one of the best T vectors for cloning of PCR products. Cloning, PCR products, pGEM-5zf(+), T vector 37 39 https://www.ajmb.org/En/Article.aspx?id=6 https://www.ajmb.org/PDF/En/FullText/6.pdf YaofengZhaoDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden19ZhancaiLiuDepartment of Physics, Chemistry and Biology, Jiaozuo Teachers College, Jiaozuo, 454001 , Henan, P. R. China20ShuyangYuDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden21SichengWenDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden22LennartHammarstromDivision of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , Stockholm, Sweden23HodjattallahRabbaniDepartment of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, Iran24