en 35509360 <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span><span style="font-size:10.0pt"> Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.</span></span></p> Breast cancer, Flow cytometry, Monoclonal antibody, Sortilin 37 45 https://www.ajmb.org/En/Article.aspx?id=40492 https://www.ajmb.org/PDF/En/FullText/40492.pdf MiganooshSimonianDepartment of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran71823MozhanHaji GhaffariDepartment of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran71824AliSalimiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran352EbrahimMirzadeganNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran653NiloufarSadeghiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran41592NasimEbrahimnezhadMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran71827GhazalehFazliMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran71828RaminaFatemiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran71829Ali AhmadBayat Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran835SaeidehMilaniBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran21487BabakNegahdari71831HodjattallahRabbani24