en 34900146 <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> The inhibitory effect of selenium nanoparticles (SeNPs) on cancer cells has been reported in many studies. In this study,&nbsp; the purpose was to compare the <em>in vitro</em> effects of SeNPs and calcium sulfate coated selenium nanoparticles (CaSO₄@ SeNPs) on breast cancer cells. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> CaSO₄@SeNPs and SeNPs were chemically synthesized and characterized with Field Emission Scanning Electron Microscope (FESEM) and energy-dispersive X-ray spectroscopy (EDX). By applying MTT assay, the cytotoxicity effect of both nanomaterials on the 4T1 cancer cells was investigated. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span> <span style="font-size:10.0pt">While LD₅₀ of SeNPs on 4T1 cancer cells was 80</span> <em><span style="font-size:10.0pt">&micro;g</span></em><span style="font-size:10.0pt">, the LD₅₀ of </span><span style="font-size:10.0pt">CaSO₄@SeNPs was </span><span style="font-size:10.0pt">reported to be only 15</span> <em><span style="font-size:10.0pt">&micro;g</span></em><span style="font-size:10.0pt">. </span><span style="font-size:10.0pt">The difference between the inhibition rates obtained for SeNPs and CaSO₄@SeNPs was statistically significant (p=0.05). </span><span style="font-size:10.0pt">In addition, at higher concentrations (50 <em>&micro;g</em>) of CaSO₄@SeNPs, the cytotoxicity was 100% more than SeNPs alone.&nbsp;</span></span></p> <p style="text-align:justify"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> According to the result of the present work, it can be concluded that decoration of SeNPs with calcium sulfate leads to an increase in potency by decreasing the effective dose. This effect can be attributed to activation of intrinsic apoptosis signaling and/or pH regulatory properties of </span><span style="font-size:10.0pt">CaSO₄@SeNPs.</span><span style="font-size:10.0pt"> However, </span><span style="font-size:10.0pt">further studies are still needed to determine the exact corresponding mechanisms of this synergistic effect.</span></p> Apoptosis, Breast neoplasms, Calcium sulfate, Nanoparticles, Selenium 201 206 https://www.ajmb.org/En/Article.aspx?id=40478 https://www.ajmb.org/PDF/En/FullText/40478.pdf ElnazFaghfuri Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran71777RamakAjidehBiotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran71778FaranakShahverdi Recombinant Vaccine Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran71779MinaHosseiniDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran71780FaranakMavandadnejadDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran71781Mohammad HosseinYazdiDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran534Ahmad RezaShahverdiRecombinant Vaccine Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran59