en 33680372 <p><span style="font-size:11pt">Background: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using &beta;-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. </span></p> <p><span style="font-size:11pt">Methods: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. </span></p> <p><span style="font-size:11pt">Results: &beta;-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. </span></p> <p><span style="font-size:11pt">Conclusion: Our present result suggests that &beta;-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis <em>in vivo</em>.</span></p> Apoptosis, Colon neoplasms, HT29 cells, Silver nanoparticles, β-sitosterol 42 46 https://www.ajmb.org/En/Article.aspx?id=40446 https://www.ajmb.org/PDF/En/FullText/40446.pdf PalaniappanChithambara ShathvihaDepartment of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India61683DevarajEzhilarasan61684ShanmugamRajeshkumarDepartment of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai,, Tamil Nadu, India61685JayaramanSelvarajDepartment of Biochemistry, Saveetha Dental College (SDC), Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India61686