en 23407796 We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays. Antibodies, Blotting, Immunohistochemistry, Monoclonal, Nestin, Peptides, Western 69 77 https://www.ajmb.org/En/Article.aspx?id=32 https://www.ajmb.org/PDF/En/FullText/32.pdf RezaHadaviMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran111Amir-HassanZarnaniNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran7NegahAhmadvandMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran112Ahmad RezaMahmoudiMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran33Ali AhmadBayatMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran12JafarMahmoudianMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran114Mohammad RezaSadeghiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran40HalehSoltanghoraeeReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran39Mohammad MehdiAkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran13MajidTarahomiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran38MahmoodJeddi-TehraniMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran15HodjattallahRabbaniImmune and Gene Therapy Lab, CCK, Department of Oncology-Pathology, Karolinska University Hospital Solna, Karolinska Institutet , Stockholm, Sweden24