en 29090070 <p>Background: The human <em>OCT4</em> gene, responsible for pluripotency and self-renewal of Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells, can generate several tran-scripts (OCT4A, OCT4B-variant 2, OCT4B-variant 3, OCT4B-variant 5, OCT4B1, OCT4 B2 and OCT4B3) by alternative splicing and alternative promoters. OCT4A that is responsible for ES and EC cell stemness properties is transcribed from a promoter upstream of Exon1a in those cells. The OCT4B group variants (OCT4B-variant2, OCT4B-variant3, OCT4B-variant5, OCT4B1, OCT4B2 and OCT4B3) are transcribed from a different promoter located in intron 1 and some of them respond to the cell stresses, but cannot sustain the ES/EC cell self-renewal. However, the exact function of OCT4B group variants is still unclear.<br /> Methods: In the present study, we employed RT-PCR and sequencing approaches to explore different forms of <em>OCT4</em> transcripts.<br /> Results: Our data revealed that the OCT4B group variants (OCT4B-variant2, OCT4 B-variant3, OCT4B1, OCT4B2 and OCT4B3) have longer 5&#39; UTR in the human bladder carcinoma cell line of 5637.<br /> Conclusion: These <em>OCT4</em> variants undergo alternative splicing in their 5&#39; UTR which might exert regulatory roles in transcription and translation mechanisms.</p> Alternative splicing, Genes, 5’untranslated regions 201 204 https://www.ajmb.org/En/Article.aspx?id=314 https://www.ajmb.org/PDF/En/FullText/314.pdf Ensieh M.PoursaniDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1139MajidMehravarDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1140AlirezaShahryariDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1259Seyed JavadMowlaDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran248BahramMohammad SoltaniDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran1141