en 29090066 <p>Background: Stem cell-based therapy is a novel strategy for the treatment of neuro-degenerative diseases. The transplantation of fully differentiated cells instead of stem cells in order to decrease serious adverse complications of stem cell therapy is a new idea. In this study, the effect of lithium chloride on dopaminergic differentiation of human immortalized RenVm cells was investigated in order to access a population of fully differentiated cells for transplantation in Parkinson disease.<br /> Methods: The immortalized RenVm cells were induced to dopaminergic differentiation using a neurobasal medium supplemented with N2 and different concentrations (1, 3, 6 <em>mM</em>) of Lithium Chloride (LiCl) for 4, 8 and 12 days. The efficiency of dopaminergic differentiation was evaluated using immunocytochemistry and western blot techniques for tyrosine hydroxylase and &beta;-catenin marker expression.<br /> Results: Our results indicated that LiCl can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner.<br /> Conclusion: It can be concluded that LiCl is able to facilitate dopaminergic differenti-ation of cultured cells by affecting Wnt-frizzled signaling pathway.</p> Beta catenin, Cell differentiation, Lithium, Wnt proteins 176 180 https://www.ajmb.org/En/Article.aspx?id=287 https://www.ajmb.org/PDF/En/FullText/287.pdf MitraSoleimaniDepartment of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran1155NazemGhasemiDepartment of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran1156