en 28496947 <p>Background: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by <em>Bacillus sp. ZR-5</em>, culture media compositions were optimized using &quot;one variable at a time&quot; strategy.<br /> Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated.<br /> Results: Maximum lipase activity was achieved after 24<em> hr</em> of incubation with 1.5% of glucose syrup (1600&plusmn;69.1 <em>u/mg</em>), 1% of fish powder (1238&plusmn;36.7 <em>u/mg</em>) and olive oil (1407&plusmn;2.1 <em>u/mg</em>) as low cost carbon and nitrogen sources and substrate, respectively.<br /> Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme.</p> Basillus pumilus ZR-5, Culture media, Lipase enzyme 82 86 https://www.ajmb.org/En/Article.aspx?id=271 https://www.ajmb.org/PDF/En/FullText/271.pdf SafouraSoleymaniDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran783HouriAlizadehDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran1103HosseinMohammadianDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran1104EmadRabbaniDepartment of Biology, Faculty of Basic Sciences, University of Isfahan, Isfahan, Iran1105FatemehMoazenDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran786HamidMirMohammad SadeghiDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran784ZiaedinSamsam ShariatDepartment of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran1107ZahraEtemadifarDepartment of Biology, Faculty of Basic Sciences, University of Isfahan, Isfahan, Iran1108MohammedRabbaniDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran782