en 27141269 <p>Background: The pathogenesis of nontypeable <em>Haemophilus influenzae</em> (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38-80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization.<br /> Methods: In the present study, the prevalence of <em>hmwA</em>, which encodes the HMW adhesin, was determined for a collection of 32 NTHi isolates. Restriction Fragment Length Polymorphism (RFLP) was performed to advance our understanding of <em>hmwA</em> binding sequence diversity.<br /> Results: The results demonstrated that <em>hmwA</em> was detected in 61% of NTHi isolates. According to RFLP, isolates were divided into three groups.<br /> Conclusion: Based on these observations, it is hypothesized that some strains of nontypeable <em>Haemophilus influenzae</em> infect some specific areas more than other parts.</p> Adhesins, Haemophilus influenza, HMW1 99 102 https://www.ajmb.org/En/Article.aspx?id=241 https://www.ajmb.org/PDF/En/FullText/241.pdf MiladShahini Shams abadiDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran993Seyed DavarSiadatDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran558FarzamVaziriDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran994MehdiDavariDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran827AbolfazlFatehDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran996ShahinPourazarDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran997FaridAbdolrahimiDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran998MortezaGhazanfariDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran999