en 26605013 <p>Background: CTLA-4 inhibitory signals prevent cell cycle progression and IL-2 production, leading to a halt on an ongoing immune response. CTLA4-Ig fusion proteins contain the extracellular domain of CTLA-4 and Fc fragment of human IgG antibody. In this study we aimed to fuse the <em>ctla-4</em> gene encoding the extracellular domain of CTLA-4 molecule with <em>igg1</em> gene encoding Fc region of human IgG.<br /> Methods: After primer design, PCR reaction was performed using pfu polymerase enzyme and specific primers. PCR amplified fragment was ligated into the vector containing the human <em>igg1</em> gene. The resulting fusion fragment of <em>ctla-4</em> and human <em>igg1</em> genes was ligated to pBudCE4.1 expression vector.<br /> Results: Extracellular domain of <em>ctla-4</em> gene was ligated to <em>igg1</em> gene and then <em>ctla4-ig</em> fragment was cloned into pBudCE4.1 vector. Construction of the expression vector was confirmed by restriction pattern analysis and sequencing.<br /> Conclusion: By confirming the construct, in the next step, the recombinant DNA will be used to produce CTLA4-Ig recombinant protein for the clinical uses.</p> Abatacept, CTLA-4 antigen, CTLA4-Ig, Recombinant DNA 179 181 https://www.ajmb.org/En/Article.aspx?id=224 https://www.ajmb.org/PDF/En/FullText/224.pdf MahsaYazdanpanah-Samani946ElhamMahmoudi Maymand947TayebehJahangeerfam948AbbasGhaderi949