en 25926948 <p>Background: Emergence of drug resistance has brought major problems in chemotherapy. Using nutrients in combination with chemotherapy could be beneficial for improvement of sensitivity of tumors to drug resistance. Soybean-derived isoflavones have been suggested as chemopreventive agents for certain types of cancer, particularly breast cancer. In this study, the synergistic effects of soy isoflavone extract in combination with docetaxel in murine 4T1 breast tumor model were investigated.</p> <p>Methods: In this study, mice were divided into 4 groups (15 mice per group) of control, the dietary Soy Isoflavone Extract (SIE, 100 <em>mg/kg</em> diet), the Docetaxel (DOCE, 10 <em>mg/kg</em>) injection and the combination of dietary soy isoflavone extract and intravenous docetaxel injection (DOCE+SIE). After 3 injections of docetaxel (once a week), 7 mice were sacrificed to analyze MKI67 gene and protein expressions and the rest were monitored for diet consumption, tumor growth and survival rates.</p> <p>Results: In DOCE+SIE group, diet consumption was significantly higher than DOCE group. While lifespan showed a trend towards improvement in DOCE+SIE group, no significant difference was observed among the 4 studied groups. Tumor volume was not significantly affected in treated groups. A lower but not significant MKI67 protein expression was detected in western blot in DOCE+SIE group. The mRNA expression was not significantly different among groups.</p> <p>Conclusion: The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.</p> Breast cancer, Docetaxel, Soy isoflavone extract 16 21 https://www.ajmb.org/En/Article.aspx?id=196 https://www.ajmb.org/PDF/En/FullText/196.pdf EhsanHejaziDepartment of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran847JavadNasrollahzadeh Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran848RaminaFatemiReproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran849LeilaBarzegar-Yar MohamadIMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran850KioomarsSaliminejadReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran393ZohreAmiriDepartment of Basic Sciences and Cellular and Molecular Nutrition, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran851MasoudKimiagarDepartment of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran852MohammadHoushyariDepartment of Radiation Oncology, Shohada Tajrish Hospital, Shahid Beheshti University of Medical Science, Tehran, Iran853MaryamTavakoliReproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran854FarahIdaliReproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran855