en 25414786 <p>Background: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. Methods: In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. Results: Frequency of IFN-&gamma; secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg+C/IFA groups (p&le;0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p&le;0.05). Conclusion: Analysis of induced responses revealed the Immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-&gamma; secreting splenocytes of immunized mice.</p> Cellular, Hepatitis B surface antigens, Humoral, Immunity, Methanobrevibacter 238 245 https://www.ajmb.org/En/Article.aspx?id=191 https://www.ajmb.org/PDF/En/FullText/191.pdf Mohammad RezaAghasadeghiDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran560Seyed AliDelbazDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran816Seyed MehdiSadatDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran817Seyed DavarSiadatDepartment of Microbiology, Pasteur Institute of Iran, Tehran, Iran558MehdiShafiee ArdestaniDepartment of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran818PoonehRahimiDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran819AzamBolhassaniDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran50RouhollahVahabpour RoudsariDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran821GolnazBahramaliDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran824FatemeMotevalliDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran826MehdiDavariDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran827HabibVakilyDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran828AliSharifat SalmaniDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran829MaryamBorhan NobariDepartment of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran830