en 31380001 <p>Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. <em>Brucella melitensis (B. mellitensis) bp26</em> gene is a good candidate for brucellosis vaccine and investigations showed that<em> Lactococcus lactis (L. lactis)</em> with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive.<br /> Methods: <em>B. mellitensis</em> <em>bp26</em> gene was cloned in food grade pNZ 8149 vector and expressed in <em>L. lactis</em> NZ 3900.<br /> Results: Results showed that we can produce a food-grade recombinant <em>L. lactis </em>producing the <em>B. melitensis </em>BP26 protein.&nbsp;<br /> Conclusion: In this study, for Future evaluation about ability of L. lactis as a live delivery vector, a food-grade recombinant <em>L. lactis</em> producing the <em>B. melitensis</em> BP26 protein was produced.&nbsp;</p> Brucellosis, Exotoxins, Lactococcus lactis, Vaccines 264 267 https://www.ajmb.org/En/Article.aspx?id=10385 https://www.ajmb.org/PDF/En/FullText/10385.pdf MaryamAzizpour MaghvanDepartment of Microbiology, Islamic Azad University, Arak Branch, Arak, Iran31494ParvanehJafariDepartment of Microbiology, Islamic Azad University, Arak Branch, Arak, Iran1163Seyyed DavoodHosseiniRazi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization, Arak, Iran1162Ali mohammadBehrozikhahRazi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization, Karaj, Iran31497