en 31057721 <p>Background: <em>Acinetobacter baumannii (A. baumannii) </em>is a bothersome fatal patho-gen, particularly in healthcare system. Persistence and successful invasion of <em>A. baumannii </em>in vertebrate host cells largely depends on iron acquisition methods. Sidero-phore molecules and Iron-Regulated Outer Membrane Proteins (IROMPs) are the two essential members of iron acquisition system. Siderophores are secreted by bacteria to bind peripheral ferric iron and the IROMPs are expressed at the bacterial outer membrane as the receptor of ferric-siderophore complex. BauA is the corresponding siderophore receptor of <em>A. baumannii</em>. In this study, an attempt was made to assess the immunogenicity of antigenic domains of BauA which could be effective in iron uptake restriction and protection against bacterial invasion of the host cells.&nbsp;<br /> Methods: The antigenic domains of <em>bauA</em> were amplified from <em>A. baumannii </em>ATCC-19606. The PCR products were ligated into pET32a and expressed in<em> Escherichia coli (E. coli)</em> BL21 (DE3). Purification of recombinant domains was done by Nickel-Nitri-lotriacetic Acid (Ni-NTA) affinity chromatography. The recombinant domains were injected into BALB/C mice separately and in combination. Sero-reactivities of the recombinant proteins and mouse challenge tests were carried out.&nbsp;<br /> Results: The antibodies raised in mice could successfully recognize and bind antigenic domains. Passive immunization studies accomplished by immune rabbit serum inhibited the establishment of infection in mice.<br /> Conclusion: The results adapted from the present study disclose the protective role of functional domains of BauA, especially the cork domain, suggesting a novel recombinant immunogen candidate.</p> Acinetobackr baumannii, Immune sera, Iron, Siderophore 180 186 https://www.ajmb.org/En/Article.aspx?id=10367 https://www.ajmb.org/PDF/En/FullText/10367.pdf HamidEsmaeilkhaniMolecular Microbiology Research Center, Shahed University, Tehran, Iran11426IrajRasooliMolecular Microbiology Research Center, Shahed University, Tehran, Iran11427MasoomehHashemiDepartment of Biology, Shahed University, Tehran, Iran11428ShahramNazarianDepartment of Biology, College of Basic Sciences, Imam Hussein University, Tehran, Iran11429FatemehSefidDeparteman of Biology, Science and Art University, Yazd, Iran11430