en 30090216 <p>Background: Disruption of the cholinergic neurotransmitter pathway which is important for cognition, memory and learning abilities has been reported in Alzheimer&rsquo;s Disease (AD) patients. The receptors involved include the Cholinergic Receptors Muscarinic (<em>CHRM</em>). <em>CHRM2</em> gene has been associated with intelligence, personality traits, substance dependence and depression. <em>CHRM3</em> has been found to heterodimerize with <em>CHRM2</em>.<br /> Methods: DNA samples from 240 AD patients with SNPs rs6962027 of <em>CHRM2</em> gene and rs7511970 of <em>CHRM3</em> gene were amplified using PCR and genotyped using Restriction Fragment Length Polymorphism (RFLP). Chi-squared test was done to check if the genes are in Hardy-Weinberg equilibrium.&nbsp;<br /> Results and Conclusion: Although the results did not show significant associations, these data denote plausible interaction between TT in SNP rs6962027 in <em>CHRM2 </em>gene and TT in SNP rs7511970 in CHRM3 gene affecting AD risk. SNP rs7511970 of <em>CHRM3</em> gene may also exert an influence on late-onset AD.</p> Alzheimer disease, Genes, Genetic polymorphism 196 199 https://www.ajmb.org/En/Article.aspx?id=10347 https://www.ajmb.org/PDF/En/FullText/10347.pdf LimYa CheePengiran Anak Puteri Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, BE 1410, Gadong, Brunei Darussalam11371AlaistairCummingPengiran Anak Puteri Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, BE 1410 , Gadong, Brunei Darussalam11370