Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb70618

The Quality and Quantity of Nanoparticles Extracted from Human Adipose Tissue Derived-Mesenchymal Stem Cells

Karimi

Mobina

Heidari

Banafsheh

Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran

Jafary

Hanieh

Zandsalimi

Kavosh

17 3 186 195 15 2 2025 19 5 2025

Background: Nanoparticles, as small extracellular vesicles, are considered promising tools in tissue engineering and regenerative medicine. This study aimed to investigate the effects of different processing and culture condition on the quality and quantity of extracts derived from human Adipose-Mesenchymal Stem Cells (AD-MSCs). Methods: AD-MSCs were proliferated in both the experimental and control groups. Nanoparticles were extracted from AD-MSCs-extracts and analyzed using SEM, TEM, DLS, Zeta potential, FTIR and BCA analyses. The morphological characteristics (shape, size, distribution, surface topography, and  agglomeration/aggregation), structural appearance (poly-disperse intensity, colloidal particle behavior, surface charge, and stability), chemical properties (functional groups and ionic interactions) and total protein concentration were detected in the extracted nanoparticles. Additionally, the morphological characteristics, apoptosis, mitochondrial oxidoreductase activity, and migration potential of AD-MSCs in both groups were evaluated using acridine orange staining, MTT, and scratch assays. Results: In the experimental group, 100% of the nanoparticles had a diameter of 112.8±25 nm, with the most frequency of 111.4 nm. However, in the control group, 72% of nanoparticles had a diameter of 350.2±43.6 nm with the highest frequency of 339.8 nm (p≤0.05). The Z-average, Poly-disperse intensity, and electrostatic stability of nanoparticles in the control and experimental groups were 171.9 nm, 0.727 and -0.000011 cm2/Vs vs. 103.7 nm, 0.205 and 0.000481 cm2/Vs, respectively (p≤0.05). In the experimental group, Zeta potential was -61.8 mV, which is in the range of ζ >-30mV. Although, Zeta potential in the control group was -1.5 mV, which is in the range of -30 mV <ζ <30 mV (p≤0.05). Total protein concentrations in the control and experimental groups were 11 and 41%, respectively (p≤0.05). Conclusion: Nanoparticles derived from AD-MSCs have high therapeutic applications in tissue engineering and regenerative medicine.