<p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Background:</span></strong><em><span style="font-size:10.0pt"> Teucrium persicum</span></em><span style="font-size:10.0pt"> (<em>T. persicum</em>) is a well-known Iranian endemic plant that grows in the southern regions of Iran. It is used as a tea to treat abdominal pains, hyperlipidemia, and diabetes in traditional Iranian medicine. It has been previously found that the methanolic extract of <em>T. persicum </em>exerts significant cytotoxicity and inhibitory effects on different cancer cells. This study aimed to investigate the effects of ethyl acetate extract of <em>T. persicum </em>on MCF-7 cells. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Methods:</span></strong><span style="font-size:10.0pt"> The experiments included MTT, DAPI staining, and investigating the expression of <em>BAX</em> and <em>BCL2</em> genes. The extract had a significant cytotoxic effect on MCF-7 cells, with an IC₅₀ value of 50 <em>&micro;g/ml</em> for 48 <em>hr</em>. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Results:</span></strong><span style="font-size:10.0pt"> DAPI staining assays showed that the extract induced morphological changes, chromatin condensation, and nuclear fragmentation. Additionally, the ethyl acetate extract induced the expression of <em>BAX</em> and down-regulated <em>BCL2</em> genes. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><strong><span style="font-size:10.0pt">Conclusion:</span></strong><span style="font-size:10.0pt"> These findings suggest that <em>T. persicum</em> has strong cytotoxic properties and warrants further investigation.</span></span></p>