Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb60544 Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias Gholami ParastouAsgarian-OmranHosseinDepartment of Agriculture Biotechnology, College of Agriculture , Tikamgarh, IndiaYaghmaeiMarjanMahmoudianJafarReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , Tehran, IranKianersiShirinSalari SinaZaboliEhsanJeddi-TehraniMahmoodMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, IranZarnaniAmir-HassanDepartment of Immunology, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , Tehran, IranShabaniMahdiDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran 15 3 167 172 24 9 2022 26 4 2023

<p><span style="font-size:11pt">Background: Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of PLAC1 in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL). </span></p> <p><span style="font-size:11pt">Methods: In this study, we investigated expression pattern of PLAC1 gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting. </span></p> <p><span style="font-size:11pt">Results: Our data demonstrated that PLAC1 transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. PLAC1 transcript expression was totally negative in all studied normal subjects. Level of PLAC1 mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of PLAC1 in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of PLAC1 in AML subtypes was not statistically significant (p=0.756). PLAC1 gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples. </span></p> <p><span style="font-size:11pt">Conclusion: Considering PLAC1 expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future.</span></p>