Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb60520

Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds

Etemadinia

Akram

Seyfoori

Amir

Rahimi Foroushani

Abbas

Mazaheri Nezhad Fard

Ramin

Bakhtiari

Ronak

14 4 317 320 6 4 2022 30 7 2022

Background: Biofilm formation helps Pseudomonas &lrm;aeruginosa (P. &lrm;aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropyl-acrylamide microgels (PNIPAM) on P. aeruginosa. Methods: Totally&lrm;, &lrm;100 P. aeruginosa strains were isolated from chronic wound infections&lrm;. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates. Results: Significant decreases were seen in lasR expression in EDTA-treated samples&lrm;. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased &lrm;expression of PA2714 was seen in samples treated with xylitol with no significance.  Conclusion: The PNIPAM containing xylitol &lrm;or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.