Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb60515 A Panel of Circulating microRNAs as a Potential Biomarker for the Early Detection of Gastric Cancer SaliminejadKioomarsDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranMahmoodzadehHabibollahSoleymani FardShahrzadDepartment of Pharmacognosy, Sri K.V.College of Pharmacy, Chickballapur , Karnataka, IndiaYaghmaeiMarjanDepartment of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranKhorram KhorshidHamid RezaMolecular Immunology and Vaccine Research Laboratory, Pasteur Institute of Iran , Tehran, IranMousaviSeyed AsadollahVaezi MohammadGhaffariSeyed Hamidollah 14 4 278 286 13 10 2021 2 7 2022

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> The high mortality rate of Gastric Cancer (GC) is a consequence of delayed diagnosis. The early diagnosis of GC could increase the five-year survival rate among patients. We aimed to find a panel of microRNAs (miRNA) for the detection of GC in the early stages. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> In this case-control study, we selected consistently upregulated miRNAs from the results of 12 high-throughput miRNA profiling studies in GC. In the profiling phase, the differential expressions of 13 candidate miRNAs were analyzed by quantitative reverse-transcription PCR (qRT-PCR) in two pooled RNA samples prepared from the plasma of eight GC patients and eight matched controls. In the validation phase, significantly upregulated miRNAs from the profiling phase were further evaluated in the plasma samples of 97 patients with stage I-IV gastric adenocarcinoma and 100 healthy controls.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span><span style="font-size:10.0pt"> In the profiling phase, six miRNAs (miR-18a, 21, 25, 92a, 125b and 221) were significantly upregulated in the GC patients compared to the controls (p&lt;0.05). However, in the validation phase, only significant up-regulation of miR-18a, 21 and 125b was confirmed (p&lt;0.05). A panel of miR-18a/21/125b was able to detect GC patients with stage I-IV from the controls (p&lt;0.001; AUC=0.92, sensitivity=86%; specificity=85%). In addition, the panel could distinguish the early-stage GC (I+II) from the control group with an AUC of 0.83, a sensitivity of 83%, and a specificity of 75%.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> A panel of circulating miR18a/21/125b could be suggested as a potential biomarker for the early detection of GC. </span></span></p>