Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb60512 Impact of Single Nucleotide Polymorphism in the ANKRD55 Gene on Occurrence and Clinical Characteristics of Rheumatoid Arthritis SalehiRasoulMotaghiMinaSalehiAmirhosseinKarimzadehHadiPakzadBahram 14 3 259 263 13 7 2022 13 7 2022

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Rheumatoid Arthritis (RA) has multifactorial etiology and numerous genetic and environmental factors have been related to an increased risk of RA. Recently, Genome-Wide Association Studies (GWAS) suggested a large number of Single Nucleotide Polymorphisms (SNPs) loci affecting the susceptibility to RA. One of these loci is rs6859219 (C&gt;A), a functional polymorphism in the <em>ANKRD55</em> gene which was associated with the expression of <em>ANKRD55</em> and <em>IL6ST</em>. In the current study, we evaluated the possible association between rs6859219 (intronic variant) in the <em>ANKRD55</em> gene with RA risk in the Iranian population.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> A case-control study using 118 RA patients and 115 healthy counterparts was undertaken in order to determine rs6859219 genotypes using real</span><span style="font-size:10.0pt">‑</span><span style="font-size:10.0pt">time polymerase chain reaction High</span><span style="font-size:10.0pt">‑</span><span style="font-size:10.0pt">Resolution Melting (HRM) method. </span></span></p> <p><span style="font-size:9.5pt">Results:</span><span style="font-size:10.0pt"> There was a significant difference in the genotype and allele frequencies of rs6859219 between patients and controls (p&lt;0.001). Logistic regression analysis demonstrates that CC genotype and C allele increased the risk of RA (OR <sub>for CC genotype</sub>= 7.12; 95%CI [3.51-15.05]/ OR <sub>for C allele</sub>=4.16; 95%CI [2.78-6.28]). Furthermore, regarding the dominant and recessive model of inheritance, RA patients indicated obvious association of the rs6859219 variant compared to healthy controls (p&lt;0.001). Moreover, in the patient group, there was a significant correlation between <span style="background-color:white">C-Reactive Protein (CRP) </span>concentration with rs6859219 polymorphism (p&lt;0.001).</span></p> <p><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> Our findings propose a substantial correlation between rs6859219 polymorphism and RA risk and clinical characteristics of this disease in the Iranian population.</span></p>