Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb60499 Association of Aberrant Promoter Methylation Changes in the Suppressor of Cytokine Signaling 3 (SOCS3) Gene with Susceptibility to Crohn's Disease SanatiGolshidJafariDavoodNoruziniaMehrdadResearch and Production plant, Pasteur Institute of Iran , Karaj, IranEbrahimi-DaryaniNaserMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, IranDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences , Tehran, IranAhmadvandMohammadTeimourianShahramRezaeiNimaBiotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran , Tehran, Iran 14 2 165 169 27 11 2021 30 1 2022

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Growing evidence supports that changes in the methylation state of Inflammatory Bowel Disease (IBD)-associated genes could significantly alter levels of gene expression, potentially contributing to disease onset and progression. We supposed that alterations in DNA methylation status at promoter region within the suppressor of cytokine signaling 3 <em>(SOCS3)</em> gene in intestinal tissues may be involved in the susceptibility to Crohn&#39;s Disease (CD).</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> DNA methylation status in the promoter region of the human <em>SOCS3</em> gene of intestinal tissues from 15 patients with CD and 15 age- and sex-matched healthy controls were profiled using the real-time Quantitative Multiplex Methylation Specific PCR (QM-MSP) assay.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span><span style="font-size:10.0pt"> Based on methylation assay data profiling, we found that patients with CD showed a higher degree of methylation of the <em>SOCS3</em> gene promoter region than did the healthy controls (unmethylated DNA in CD <em>vs.</em> healthy controls; 0.00048&plusmn;0.0011 <em>vs.</em> 0.07&plusmn;0.142, p&lt;0.000).</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> The data presented here demonstrate that aberrant methylation of the CpG islands within promoter regions of <em>SOCS3</em> gene in colonic mucosa of CD was associated with mucosal inflammatory status, providing insights into the involvement of methylation could contribute to the initiation of the inflammatory process and development of CD.</span></span></p>