Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb40492 Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells SimonianMiganooshHaji GhaffariMozhanSalimiAliReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranMirzadeganEbrahimDepartment of Bacteriology, Pasteur Institute of Iran, Tehran, IranSadeghiNiloufarEbrahimnezhadNasimFazliGhazalehFatemiRaminaBayat Ali AhmadMilaniSaeidehNegahdariBabakRabbaniHodjattallahImmune and Gene Therapy Laboratory, CCK, Karolinska University Hospital Solna , Stockholm, SwedenDepartment of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, Iran 14 1 37 45 12 7 2021 25 9 2021

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Background:</span><span style="font-size:10.0pt"> Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Methods:</span><span style="font-size:10.0pt"> In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. </span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Results:</span><span style="font-size:10.0pt"> Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.</span></span></p> <p style="text-align:justify"><span style="font-size:11pt"><span style="font-size:9.5pt">Conclusion:</span><span style="font-size:10.0pt"> Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.</span></span></p>