<p><span style="font-size:11pt">Background: The present study aimed to investigate the antifungal activity of Nanoparticles (NPs) against amphotericin B-resistant <em>Candida glabrata</em> (<em>C. glabrata</em>) strains. </span></p> <p><span style="font-size:11pt">Methods: Twelve resistant (<em>C. glabrata</em>) strains were isolated from archived clinical isolates. Antifungal activity was conducted according to Clinical and Laboratory Standards Institute&rsquo;s (CLSI) guidelines, document M27-A3/S4. The Scanning Electron Microscope (SEM) was used to observe the morphological changes of strains exposed to each nanoparticle. </span></p> <p><span style="font-size:11pt">Results: Minimum Inhibitory Concentration (MIC) of nanoparticles of all strains was in the concentration range of 0.125 to 0.5 <em>&micro;g/Ml</em>. The synthesized Ag-NPs showed superior antifungal activity against (<em>C. glabrata</em>) compared to Se-NPs and Au-NPs. The scanning electron microscope images revealed the difference in the fungal morphology between the untreated and treated fungi with nanoparticles. </span></p> <p><span style="font-size:11pt">Conclusion: The Ag-NPs, followed by Se-NPs synthesized, revealed significant antifungal activity against resistance regardless of their antifungal-resistant mechanisms.</span></p>