Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb334 Assessment of <i>EGFR</i> Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos GazorRouhollahEskandariMozhganSharafshahAlirezaBahadoriMohammad HadiGolmohammadiMohammad GhasemKeshavarzParvanehDrug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran 10 2 120 122 29 5 2017 4 9 2016

<p>Background: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal <em>Growth Factor Receptor</em> (<em>EGFR</em>) gene in mouse 2-cell and blastocysts.<br /> Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44-46 and 88-89 <em>hr</em>, respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their morphology and their RNA was extracted. Quantitative expression of <em>EGFR</em> gene in both groups was investigated by applying real time-PCR.<br /> Results: The statistical real-time (RT)-PCR analyses performed using SPSS revealed that the expression level of EGFR gene was diminished in the case group compared to the control group.&nbsp;<br /> Conclusion: The current study indicated the negative effect of cryopreservation on expression amount of <em>EGFR</em> gene in 2-cell and blastocyst mouse embryos.</p>