<p>Background: The purpose of this study was to investigate the sterol profiling and predict the pharmacological potential of marine gastropod <em>Telescopium telescopium (T. telescopium)</em>, collected from mangrove ecosystem in the South west coast of India.<br /> Methods: Sterol fractions were separated from the crude lipids using 15% ethyl acetate. Ethyl acetate fractions were dried under ultrahigh purity N₂ and analyzed using GC-MS. The biological activity was predicted using the software CLC-Pred; <em>In silico</em> predictions of cytotoxicity for tumor and non-tumor cell lines and PASS.&nbsp;<br /> Results: This study proved the existence of four sterols, of which cholesterol was abundant. It was found that most of the steroids profiled from <em>T. telescopium</em> displayed activity against reproductive system as well as skin related diseases.&nbsp;<br /> Conclusion: The predicted anti infertility and skin related activity of the steroids identified from the marine gastropod <em>T. telescopium</em> is useful to attract industrial interest towards this species which will be helpful in rising new combinations with added therapeutic and nutritional worth.</p>