Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb309

Tumor Necrosis Factor-Alpha and Interleukin-6 Gene Polymorphisms in Iranian Patients with Ischemic Heart Failure

Hedayat

Mona

Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Mahmoudi

Mohammad Jafar

Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Clinical Biochemistry, Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Taghvaei

Mohammad

Department of Biotechnology, College of Science, Tehran University, Tehran, Iran

Nematipour

Ebrahim

Department of Biology, Shahed University, Tehran, Iran

Farhadi

Elham

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Esfahanian

Nilufar

Department of Biology, Shahed University, Tehran, Iran

Mahmoudi

Maryam

Department of Biology, Shahed University, Tehran, Iran

Sadr

Maryam

Department of Biology, Research Center for Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Nourijelyani

Keramat

Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Amirzargar

Ali Akbar

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, IranDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran

Rezaei

Nima

Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran , Tehran, Iran

10 2 105 109 30 1 2017 8 5 2017

Background: Proinflammatory cytokines have been known to be elevated in patients with Chronic Heart Failure (CHF). Given the importance of proinflammatory cytokines in the context of the failing heart, the prevalence of Tumor Necrosis Factor-α (TNF-α), Interleukin (IL)-6 polymorphisms in patients with CHF was studied due to ischemic heart disease. Methods: Forty three patients with ischemic heart failure were enrolled in this study and compared with 140 healthy individuals. The allele and genotype frequency of four Single Nucleotide Polymorphisms (SNPs) within the IL-6 (-174, nt565) and TNF-α (-308, -238) genes were determined, using Polymerase Chain Reaction with Sequence-Specific Primers (PCR-SSP) assay. Results: The frequency of the TNF-α (-238) A/A genotype was significantly higher in patients comparing to controls (p=0.043), while TNF-α G/A genotype at the same position decreased significantly, in comparison with controls (p=0.018). The most frequent haplotype for TNF-α was A/A in the patient group in comparison with controls (p=0.003). There was no significant difference in allele and genotype frequencies of IL-6 at positions -174 and nt565, and TNF-α at position -308. Conclusion: Certain alleles, genotypes, and haplotypes in TNF-α, but not IL-6, gene were overrepresented in patients with ischemic heart failure, which may, in turn, predispose individuals to this disease.