Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb271

Efficient Media for High Lipase Production: One Variable at a Time Approach

Soleymani

Safoura

Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Alizadeh

Houri

Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Mohammadian

Hossein

Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Rabbani

Emad

Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran

Moazen

Fatemeh

Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

MirMohammad Sadeghi

Hamid

Immunology Research Center (IRC), Tabriz University of Medical Science, Tabriz, Iran

Samsam Shariat

Ziaedin

Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Etemadifar

Zahra

Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Rabbani

Mohammed

Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

9 2 82 86 12 3 2016 25 6 2016

Background: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by Bacillus sp. ZR-5, culture media compositions were optimized using "one variable at a time" strategy. Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated. Results: Maximum lipase activity was achieved after 24 hr of incubation with 1.5% of glucose syrup (1600±69.1 u/mg), 1% of fish powder (1238±36.7 u/mg) and olive oil (1407±2.1 u/mg) as low cost carbon and nitrogen sources and substrate, respectively. Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme.